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RPA 32 kDa subunit Antibody (MA34): sc-53496

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Datasheets
  • RPA 32 kDa subunit Antibody (MA34) is a mouse monoclonal IgG1 κ, cited in 12 publications, provided at 200 µg/ml
  • raised against RPA 32 kDa subunit of human origin
  • recommended for detection of RPA 32 kDa subunit of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • available conjugated to Alexa Fluor® 647 (sc-53496 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See RPA 32 kDa subunit (9H8): sc-56770 for RPA 32 kDa subunit antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RPA 32 kDa subunit Antibody (MA34) for WB and IHC(P) applications. These reagents are now offered in bundles with RPA 32 kDa subunit Antibody (MA34) (see ordering information below).

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RPA 32 kDa subunit Antibody (MA34) is a mouse monoclonal IgG1 antibody that detects RPA 32 kDa subunit in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). RPA 32 kDa subunit is a critical component of the replication protein A complex, which is essential for maintaining genomic stability by facilitating DNA replication, repair, and recombination processes. RPA 32 kDa subunit is primarily located in the nucleus, where RPA 32 kDa subunit binds to single-stranded DNA, thereby preventing the formation of secondary structures that could impede replication and repair mechanisms. RPA 32′s ability to interact with various DNA repair enzymes, such as UNG2, XPA, and Rad52, underscores RPA 32′s importance in the nucleotide excision repair pathway, which is vital for correcting DNA damage caused by environmental factors. Additionally, RPA 32′s interaction with the SH2 domain of Stat3 highlights RPA 32′s role in cellular signaling pathways, where RPA 32 can influence growth factor-stimulated transcriptional activities. Anti-RPA 32 kDa subunit antibody (MA34)′s versatility in various applications makes anti-RPA 32 kDa subunit antibody (MA34) an invaluable tool for researchers studying DNA repair mechanisms and cellular signaling in different species.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

RPA 32 kDa subunit Antibody (MA34)

sc-53496
200 µg/ml
$316.00

RPA 32 kDa subunit Antibody (MA34): m-IgG Fc BP-HRP Bundle

sc-536973
200 µg Ab; 10 µg BP
$354.00

RPA 32 kDa subunit Antibody (MA34): m-IgGκ BP-HRP Bundle

sc-534150
200 µg Ab; 40 µg BP
$354.00

RPA 32 kDa subunit Antibody (MA34): m-IgG1 BP-HRP Bundle

sc-544933
200 µg Ab; 20 µg BP
$354.00

RPA 32 kDa subunit Antibody (MA34) Alexa Fluor® 647

sc-53496 AF647
200 µg/ml
$357.00

Can RPA 32 kDa subunit (MA34): sc-53496 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, RPA 32 kDa subunit (MA34): sc-53496 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_12, d_14, h_10CST
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Rated 4 out of 5 by from Strong bands seen in WB using HeLaStrong bands seen in WB using HeLa, T-47D, MCF7 and Saos-2 whole cell lysates and Ramos and HeLa nuclear extracts. -SCBT QC
Date published: 2015-06-25
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human lung cancer cell lines A549 and H719 + cells treated with knockdown of DNMTs or 5-aza-CdR and 8226-LR5 cells -SCBT Publication Review
Date published: 2015-05-21
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2014-11-13
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RPA 32 kDa subunit Antibody (MA34) is rated 4.0 out of 5 by 3.
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