RPA 32 kDa subunit Antibody (9H8): sc-56770
- RPA 32 kDa subunit Antibody (9H8) is a mouse monoclonal IgG1 κ RPA 32 kDa subunit antibody, cited in 43 publications, provided at 200 µg/ml
- raised against full length RPA 32 kDa subunit of human origin
- RPA 32 kDa subunit Antibody (9H8) is recommended for detection of RPA 32 kDa subunit of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-RPA 32 kDa subunit Antibody (9H8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RPA 32 kDa subunit Antibody (9H8) for WB and IHC(P) applications. These reagents are now offered in bundles with RPA 32 kDa subunit Antibody (9H8) (see ordering information below).
QUICK LINKS
SEE ALSO...
RPA 32 kDa subunit Antibody (9H8) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPA 32 kDa subunit protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RPA 32 kDa subunit (9H8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPA 32 kDa subunit is a critical component of the replication protein A (RPA) complex, which is essential for DNA replication, recombination, and repair in eukaryotic cells. RPA 32 kDa subunit binds to single-stranded DNA in the nucleus, stabilizing DNA and preventing secondary structures that could impede replication and repair processes. Nuclear localization of RPA 32 kDa subunit allows efficient access to DNA during critical cellular processes, ensuring genomic stability and integrity. RPA 32 kDa subunit interacts with various proteins involved in DNA repair, including UNG2, XPA, and Rad52, coordinating cellular response to DNA damage. Specific binding of RPA 32 kDa subunit to the SH2 domain of Stat3 suggests involvement in signaling pathways that regulate cell growth and survival, making RPA 32 kDa subunit significant in both DNA maintenance and cellular signaling.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPA 32 kDa subunit Antibody (9H8) References:
- Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance. | Smith, J., et al. 2000. Biochimie. 82: 71-8. PMID: 10717390
- RPA stabilizes the XPA-damaged DNA complex through protein-protein interaction. | Wang, M., et al. 2000. Biochemistry. 39: 6433-9. PMID: 10828957
- Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity. | Kim, J., et al. 2000. Cell Biol Int. 24: 467-73. PMID: 10875894
- 5' --> 3' molecular polarity of human replication protein A (hRPA) binding to pseudo-origin DNA substrates. | Iftode, C. and Borowiec, JA. 2000. Biochemistry. 39: 11970-81. PMID: 11009611
- Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. | Mer, G., et al. 2000. Cell. 103: 449-56. PMID: 11081631
- Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication. | Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
- The primary structure of the 32-kDa subunit of human replication protein A. | Erdile, LF., et al. 1990. J Biol Chem. 265: 3177-82. PMID: 2406247
- Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. | Bochkarev, A., et al. 1997. Nature. 385: 176-81. PMID: 8990123
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPA 32 kDa subunit Antibody (9H8) | sc-56770 | 200 µg/ml | $322.00 | |||
RPA 32 kDa subunit Antibody (9H8): m-IgG Fc BP-HRP Bundle | sc-528620 | 200 µg Ab; 10 µg BP | $361.00 | |||
RPA 32 kDa subunit Antibody (9H8): m-IgGκ BP-HRP Bundle | sc-521044 | 200 µg Ab, 40 µg BP | $361.00 | |||
RPA 32 kDa subunit Antibody (9H8): m-IgG1 BP-HRP Bundle | sc-543032 | 200 µg Ab; 20 µg BP | $361.00 | |||
RPA 32 kDa subunit Antibody (9H8) AC | sc-56770 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RPA 32 kDa subunit Antibody (9H8) HRP | sc-56770 HRP | 200 µg/ml | $322.00 | |||
RPA 32 kDa subunit Antibody (9H8) FITC | sc-56770 FITC | 200 µg/ml | $336.00 | |||
RPA 32 kDa subunit Antibody (9H8) PE | sc-56770 PE | 200 µg/ml | $349.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 488 | sc-56770 AF488 | 200 µg/ml | $364.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 546 | sc-56770 AF546 | 200 µg/ml | $364.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 594 | sc-56770 AF594 | 200 µg/ml | $364.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 647 | sc-56770 AF647 | 200 µg/ml | $364.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 680 | sc-56770 AF680 | 200 µg/ml | $364.00 | |||
RPA 32 kDa subunit Antibody (9H8) Alexa Fluor® 790 | sc-56770 AF790 | 200 µg/ml | $364.00 |