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RPA 14 kDa subunit Antibody (E-2) - Western Blotting - Image 147323
RPA 14 kDa subunit Antibody (E-2): sc-271564. Western blot analysis of RPA 14 kDa subunit expression in HL-60 (A) and K-562 (B) whole cell lysates and HL-60 (C) and MOLT-4 (D) nuclear extracts.
RPA 14 kDa subunit Antibody (E-2) - Western Blotting - Image 147323
RPA 14 kDa subunit Antibody (E-2): sc-271564. Western blot analysis of RPA 14 kDa subunit expression in HL-60 (A) and K-562 (B) whole cell lysates and HL-60 (C) and MOLT-4 (D) nuclear extracts.
RPA 14 kDa subunit Antibody (E-2) - Western Blotting - Image 134126
RPA 14 kDa subunit Antibody (E-2): sc-271564. Western blot analysis of RPA 14 kDa subunit expression in K-562 (A) and HL-60 (B) nuclear extracts and K-562 whole cell lysate (C).
RPA 14 kDa subunit Antibody (E-2): sc-271564. Western blot analysis of RPA 14 kDa subunit expression in HL-60 (A) and K-562 (B) whole cell lysates and HL-60 (C) and MOLT-4 (D) nuclear extracts.

RPA 14 kDa subunit Antibody (E-2): sc-271564

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Datasheets
  • RPA 14 kDa subunit Antibody (E-2) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 24-81 mapping within an internal region of RPA 14 kDa subunit of human origin
  • recommended for detection of RPA 14 kDa subunit of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RPA 14 kDa subunit Antibody (E-2) for WB applications. These reagents are now offered in bundles with RPA 14 kDa subunit Antibody (E-2) (see ordering information below).

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    SEE ALSO...

    Additional Replication Protein A Antibodies including RPA 14 kDa subunit, RPA 32 kDa subunit and RPA 70 kDa subunit

    RPA 14 kDa subunit Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPA 14 kDa subunit protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPA 14 kDa subunit (E-2) antibody is available in a non-conjugated form, allowing for versatile applications in various experimental settings. RPA 14 kDa subunit, also known as RPA3, is a critical component of the replication protein A (RPA) complex, which is essential for DNA replication, recombination, and repair processes in eukaryotic cells. RPA3 is primarily located in the nucleus, where RPA3 plays a pivotal role in maintaining genomic stability by binding to single-stranded DNA (ssDNA) during DNA damage response and repair mechanisms. The RPA complex, composed of the 14 kDa, 32 kDa, and 70 kDa subunits, acts as a damage-recognition structure in nucleotide excision repair, highlighting RPA3′s importance in cellular responses to DNA damage. RPA3′s ability to interact with ssDNA is crucial for the recruitment of other repair proteins, thereby facilitating efficient DNA repair and ensuring the integrity of the genetic material. Anti-RPA 14 kDa subunit antibody (E-2) enables researchers to gain valuable insights into the mechanisms of DNA metabolism and cellular responses to DNA damage across different species.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    RPA 14 kDa subunit Antibody (E-2) References:

    1. Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes.  |  Zou, L. and Elledge, SJ. 2003. Science. 300: 1542-8. PMID: 12791985
    2. DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells.  |  Dodson, GE., et al. 2004. J Biol Chem. 279: 34010-4. PMID: 15197179
    3. Replication protein A interacts with AID to promote deamination of somatic hypermutation targets.  |  Chaudhuri, J., et al. 2004. Nature. 430: 992-8. PMID: 15273694
    4. RPA-like proteins mediate yeast telomere function.  |  Gao, H., et al. 2007. Nat Struct Mol Biol. 14: 208-14. PMID: 17293872
    5. Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers.  |  Michiels, S., et al. 2007. Carcinogenesis. 28: 1731-9. PMID: 17494052
    6. Cellular functions of human RPA1. Multiple roles of domains in replication, repair, and checkpoints.  |  Haring, SJ., et al. 2008. J Biol Chem. 283: 19095-111. PMID: 18469000
    7. High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3).  |  Umbricht, CB., et al. 1994. Genomics. 20: 249-57. PMID: 8020972
    8. Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A.  |  Umbricht, CB., et al. 1993. J Biol Chem. 268: 6131-8. PMID: 8454588

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    RPA 14 kDa subunit Antibody (E-2)

    sc-271564
    200 µg/ml
    		$316.00

    RPA 14 kDa subunit Antibody (E-2): m-IgG Fc BP-HRP Bundle

    sc-527258
    200 µg Ab; 10 µg BP
    		$354.00

    RPA 14 kDa subunit Antibody (E-2): m-IgG1 BP-HRP Bundle

    sc-532631
    200 µg Ab; 20 µg BP
    		$354.00

    I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-271564: RPA 14 kDa subunit (E-2) mouse monoclonal antibody will work?

    Asked by: DefinitelyNotMatt
    Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
    Answered by: Technical Support
    Date published: 2017-03-06
    • y_2025, m_12, d_5, h_10CST
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    Rated 5 out of 5 by from greatI used this antiboty 1:500 for WB,the band is clear and have no background
    Date published: 2017-09-23
    Rated 5 out of 5 by from Great for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
    Date published: 2017-09-06
    Rated 4 out of 5 by from Used this in IF and got good stainingUsed this in IF and got good staining.
    Date published: 2015-09-11
    Rated 5 out of 5 by from Great Western Blot data of RPA 14 kDa subunitGreat Western Blot data of RPA 14 kDa subunit expression in HL-60 and K-562 whole cell lysates and HL-60 and MOLT-4 nuclear extracts. -SCBT QC
    Date published: 2015-04-25
    Rated 5 out of 5 by from Good Western Blot data of RPA 14 kDa subunitGood Western Blot data of RPA 14 kDa subunit expression in K-562 and HL-60 nuclear extracts and K-562 whole cell lysate . -SCBT QC
    Date published: 2013-05-27
    • y_2025, m_12, d_5, h_10
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    RPA 14 kDa subunit Antibody (E-2) is rated 4.8 out of 5 by 5.
    • y_2025, m_12, d_5, h_10
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