RP2 Antibody (C-9): sc-390220
- RP2 Antibody (C-9) is a mouse monoclonal IgG1 κ RP2 antibody provided at 200 µg/ml
- raised against amino acids 142-350 mapping at the C-terminus of RP2 of human origin
- RP2 Antibody (C-9) is recommended for detection of RP2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RP2 Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RP2 Antibody (C-9) for WB applications. These reagents are now offered in bundles with RP2 Antibody (C-9) (see ordering information below).
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RP2 Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects RP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RP2 (C-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RP2 protein, also known as retinitis pigmentosa 2 or TBCCD2, is a 350 amino acid protein that is primarily localized to the cytoplasmic side of the cell membrane and is a member of the TBCC family. RP2 protein plays a crucial role in retinal development by stimulating the GTPase activity of tubulin and acting as a guanine nucleotide dissociation inhibitor for ARL3 (ADP-ribosylation factor-like 3), which is essential for maintaining proper cellular functions. The interaction between RP2 and ARL3 is particularly important as RP2 prevents the dissociation of the GTP-bound form of ARL3, thereby regulating various cellular processes. Additionally, RP2 can undergo post-translational modifications such as myristoylation and palmitoylation, which are vital for correct membrane targeting and function. Defects in the gene encoding RP2 are linked to retinitis pigmentosa type 2, a genetic disorder that leads to the degeneration of photoreceptor cells, resulting in night vision blindness and progressive loss of both peripheral and central vision.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RP2 Antibody (C-9) References:
- Genotype-phenotype correlation in X-linked retinitis pigmentosa 2 (RP2). | Rosenberg, T., et al. 1999. Ophthalmic Genet. 20: 161-72. PMID: 10520237
- Novel frameshift mutations in the RP2 gene and polymorphic variants. | Thiselton, DL., et al. 2000. Hum Mutat. 15: 580. PMID: 10862093
- Mutations in the N-terminus of the X-linked retinitis pigmentosa protein RP2 interfere with the normal targeting of the protein to the plasma membrane. | Chapple, JP., et al. 2000. Hum Mol Genet. 9: 1919-26. PMID: 10942419
- Functional overlap between retinitis pigmentosa 2 protein and the tubulin-specific chaperone cofactor C. | Bartolini, F., et al. 2002. J Biol Chem. 277: 14629-34. PMID: 11847227
- A comprehensive mutation analysis of RP2 and RPGR in a North American cohort of families with X-linked retinitis pigmentosa. | Breuer, DK., et al. 2002. Am J Hum Genet. 70: 1545-54. PMID: 11992260
- Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3. | Kühnel, K., et al. 2006. Structure. 14: 367-78. PMID: 16472755
- Mutational analysis of RPGR and RP2 genes in Japanese patients with retinitis pigmentosa: identification of four mutations. | Jin, ZB., et al. 2006. Mol Vis. 12: 1167-74. PMID: 17093403
- Mapping the RP2 locus for X-linked retinitis pigmentosa on proximal Xp: a genetically defined 5-cM critical region and exclusion of candidate genes by physical mapping. | Thiselton, DL., et al. 1996. Genome Res. 6: 1093-102. PMID: 8938433
- Positional cloning of the gene for X-linked retinitis pigmentosa 2. | Schwahn, U., et al. 1998. Nat Genet. 19: 327-32. PMID: 9697692
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RP2 Antibody (C-9) | sc-390220 | 200 µg/ml | $322.00 | |||
RP2 Antibody (C-9): m-IgG Fc BP-HRP Bundle | sc-530098 | 200 µg Ab; 10 µg BP | $361.00 | |||
RP2 Antibody (C-9): m-IgGκ BP-HRP Bundle | sc-523454 | 200 µg Ab, 40 µg BP | $361.00 | |||
RP2 Antibody (C-9): m-IgG1 BP-HRP Bundle | sc-543869 | 200 µg Ab; 20 µg BP | $361.00 | |||
RP2 Antibody (C-9) AC | sc-390220 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RP2 Antibody (C-9) HRP | sc-390220 HRP | 200 µg/ml | $322.00 | |||
RP2 Antibody (C-9) FITC | sc-390220 FITC | 200 µg/ml | $336.00 | |||
RP2 Antibody (C-9) PE | sc-390220 PE | 200 µg/ml | $349.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 488 | sc-390220 AF488 | 200 µg/ml | $364.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 546 | sc-390220 AF546 | 200 µg/ml | $364.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 594 | sc-390220 AF594 | 200 µg/ml | $364.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 647 | sc-390220 AF647 | 200 µg/ml | $364.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 680 | sc-390220 AF680 | 200 µg/ml | $364.00 | |||
RP2 Antibody (C-9) Alexa Fluor® 790 | sc-390220 AF790 | 200 µg/ml | $364.00 |