RORβ CRISPR/Cas9 KO Plasmid (m): sc-432582
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RORβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RORβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RORβ Antibody (4B4): sc-293471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RORβ CRISPR/Cas9 KO Plasmid (m) | sc-432582 | 20 µg | $397.00 |
Overview
Rorb encodes the nuclear receptor RORβ (retinoic acid receptor-related orphan receptor beta), a ligand-regulated transcription factor that binds ROR response elements to control cell type–specific gene expression programs. In mouse, RORβ is enriched in the central nervous system and retina, where it contributes to neuronal differentiation, laminar patterning, circadian-linked transcriptional rhythms, and photoreceptor-related gene networks. As a member of the ROR/REV-ERB axis, it intersects with developmental and metabolic transcriptional pathways that shape neuronal identity and synaptic function. Altered RORβ-dependent regulation has been associated with neurodevelopmental and sensory phenotypes, supporting its use in studies of neural circuit formation and retinal biology.
RORβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rorb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rorb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rorb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RORβ protein expression.
This CRISPR knockout system enables efficient generation of Rorb-deficient cell models for investigation of RORβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rorb exon(s) critical for RORβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rorb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RORβ CRISPR/Cas9 KO Plasmid (m) and RORβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rorb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RORβ HDR Plasmid (m) and RORβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rorb homology arms to support homology-directed repair at defined Rorb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.