Rootletin CRISPR/Cas9 KO Plasmid (h): sc-401646
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rootletin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rootletin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rootletin Antibody (C-2): sc-374056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rootletin CRISPR/Cas9 KO Plasmid (h) | sc-401646 | 20 µg | $397.00 |
Overview
CROCC encodes rootletin, a large coiled-coil cytoskeletal protein that forms centrosome-associated ciliary rootlets and contributes to centrosome cohesion and structural organization. Rootletin supports basal body integrity and ciliogenesis, linking centrosomal architecture to microtubule organization and cell cycle progression. Disruption of CROCC can perturb cilia-dependent signaling networks and mitotic fidelity, processes that are frequently interrogated in studies of ciliopathies, retinal degeneration, and proliferative disease mechanisms. As a human centrosomal component, rootletin is also relevant to research on centrosome abnormalities, chromosome instability, and cell polarity.
Rootletin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CROCC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CROCC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CROCC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rootletin protein expression.
This CRISPR knockout system enables efficient generation of CROCC-deficient cell models for investigation of Rootletin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CROCC exon(s) critical for Rootletin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CROCC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rootletin CRISPR/Cas9 KO Plasmid (h) and Rootletin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CROCC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rootletin HDR Plasmid (h) and Rootletin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CROCC homology arms to support homology-directed repair at defined CROCC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.