Date published: 2026-4-5

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Rock-2 Antibody (30-J): sc-100425

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Datasheets
  • Rock-2 Antibody (30-J) is a mouse monoclonal IgG2b κ, cited in 7 publications, provided at 100 µg/ml
  • raised against recombinant Rock-2 of human origin
  • recommended for detection of Rock-2 of mouse, rat and human origin by WB, IP, IF and ELISA
  • See Rock-2 (D-11): sc-398519 for Rock-2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rock-2 Antibody (30-J). This work is in progress.
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Rock-2 Antibody (30-J) is a mouse monoclonal IgG2b antibody that detects Rock-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rock-2, also known as Rho-associated coiled-coil containing protein kinase 2, plays a crucial role in regulating various cellular processes, including cell migration, proliferation, and apoptosis, by acting as a downstream effector of the Rho GTPase signaling pathway. Rock-2 (30-J) antibody helps researchers study Rock-2′s ability to modulate the actin cytoskeleton, which is essential for maintaining cell shape and facilitating movement. Additionally, Rock-2 is involved in the phosphorylation of myosin light chains, which is critical for muscle contraction and cellular tension. Understanding the function of Rock-2 is vital, as dysregulation of Rock-2 has been implicated in various diseases, including cancer and cardiovascular disorders. Anti-Rock-2 antibody (30-J) provides a reliable tool for researchers aiming to explore the intricate roles of Rock-2 in cellular dynamics and disease mechanisms.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rock-2 Antibody (30-J)

sc-100425
100 µg/ml
$339.00

Which epitope is recognized by sc-100425 Rock-2 (30-J)?

Asked by: Anonymous
Thank you for your question. Rock-2 Antibody (30-J): sc-100425 has been raised against recombinant Rock-2 of human origin. No epitope mapping has been performed for this antibody yet, so the exact binding region of the antibody is not known. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2025-08-08

Which epitope is recognized by sc-100425 Rock-2 (30-J)?

Asked by: BG_Trieste
Thank you for your question. Rock-2 Antibody (30-J): sc-100425 has been raised against recombinant Rock-2 of human origin. No epitope mapping has been performed for this antibody yet, so the exact binding region of the antibody is not known.
Answered by: Tech Support Europe
Date published: 2019-06-05

I am using Rock-2 (30-J): sc-100425 for immunofluorescence, what dilution of this antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from nice quality.We have also used the antibody for IP of endogenous Rock-2 from a primary culture of mouse astrocytes,I got expected signal.It's very nice quality.
Date published: 2017-08-12
Rated 5 out of 5 by from Great WB results with HeLa whole cell lysateGreat WB results with HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-27
Rated 4 out of 5 by from Produced clear immunofluorescence nuclearProduced clear immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-05
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Rock-2 Antibody (30-J) is rated 4.7 out of 5 by 3.
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