Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rock-1 CRISPR/Cas9 KO Plasmid (h): sc-400367
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rock-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rock-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rock-1 Antibody (G-6): sc-17794
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rock-1 CRISPR/Cas9 KO Plasmid (h) | sc-400367 | 20 µg | $397.00 |
Overview
ROCK1 encodes Rho-associated coiled-coil containing protein kinase 1 (Rock-1), a serine/threonine kinase that functions as a principal effector of RhoA to regulate actomyosin contractility and cytoskeletal remodeling. Rock-1 phosphorylates key substrates controlling stress fiber formation, focal adhesion dynamics, and myosin light chain activity, linking Rho/ROCK signaling to cell shape, motility, adhesion, and cytokinesis. Through these processes, ROCK1 contributes to mechanotransduction and tissue architecture, and its dysregulation has been associated with altered invasive behavior and abnormal vascular and fibrotic responses in disease biology. ROCK1 is also implicated in apoptosis and inflammatory signaling contexts, making it a relevant node for dissecting pathway cross-talk in diverse cellular models.
Rock-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ROCK1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ROCK1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ROCK1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rock-1 protein expression.
This CRISPR knockout system enables efficient generation of ROCK1-deficient cell models for investigation of Rock-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ROCK1 exon(s) critical for Rock-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ROCK1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rock-1 CRISPR/Cas9 KO Plasmid (h) and Rock-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ROCK1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rock-1 HDR Plasmid (h) and Rock-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ROCK1 homology arms to support homology-directed repair at defined ROCK1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.