RNH1 Antibody (F-5): sc-376066
- RNH1 Antibody (F-5) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 1-135 mapping at the N-terminus of RNH1 of human origin
- recommended for detection of RNH1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for RNH1 Antibody (F-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with RNH1 Antibody (F-5) (see ordering information below).
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RNH1 Antibody (F-5) is a mouse monoclonal IgG2a antibody that detects RNH1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RNH1, known as the placental ribonuclease inhibitor, is an acidic protein composed of 460 amino acids, characterized by a notably high content of leucine and cysteine residues. RNH1 plays a crucial role in cellular processes by binding to a wide range of ribonucleases, effectively keeping them in a latent state and thus regulating mRNA turnover. RNH1 inhibits angiogenesis by reversibly binding to angiogenin, a member of the RNase A superfamily, highlighting its significance in cancer biology, as angiogenesis is essential for tumor growth and metastasis. By modulating the activity of RNases, RNH1 not only contributes to the maintenance of RNA stability but also presents potential therapeutic implications in cancer gene therapy, making anti-RNH1 antibody (F-5) an invaluable tool for researchers studying these critical biological processes.
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RNH1 Antibody (F-5) References:
- Superadditive and subadditive effects of 'hot spot' mutations within the interfaces of placental ribonuclease inhibitor with angiogenin and ribonuclease A. | Chen, CZ. and Shapiro, R. 1999. Biochemistry. 38: 9273-85. PMID: 10413501
- Characterisation of a tryptic peptide from human placental ribonuclease inhibitor which inhibits ribonuclease activity. | Crevel-Thieffry, I., et al. 1992. Biochim Biophys Acta. 1122: 107-12. PMID: 1633192
- Characterization of the tryptophan residues of human placental ribonuclease inhibitor and its complex with bovine pancreatic ribonuclease A by steady-state and time-resolved emission spectroscopy. | Sardar, PS., et al. 2006. J Phys Chem B. 110: 21349-56. PMID: 17048964
- Sensitivity of monomeric and dimeric forms of bovine seminal ribonuclease to human placental ribonuclease inhibitor. | Murthy, BS. and Sirdeshmukh, R. 1992. Biochem J. 281 (Pt 2): 343-8. PMID: 1736883
- Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. | Emara, MM., et al. 2010. J Biol Chem. 285: 10959-68. PMID: 20129916
- Plasma autoantibodies against heat shock protein 70, enolase 1 and ribonuclease/angiogenin inhibitor 1 as potential biomarkers for cholangiocarcinoma. | Rucksaken, R., et al. 2014. PLoS One. 9: e103259. PMID: 25058392
- Tight-binding inhibition of angiogenin and ribonuclease A by placental ribonuclease inhibitor. | Lee, FS., et al. 1989. Biochemistry. 28: 225-30. PMID: 2706246
- An in vitro binding assay for angiogenin using placental ribonuclease inhibitor. | Bond, MD. 1988. Anal Biochem. 173: 166-73. PMID: 3189795
- RNA-cDNA hybrids mediate transposition via different mechanisms. | Todd, LA., et al. 2020. Sci Rep. 10: 16034. PMID: 32994470
- Effect of human placental ribonuclease inhibitor in cell-free ribosomal RNA synthesis. | Eichler, DC., et al. 1981. Biochem Biophys Res Commun. 101: 396-403. PMID: 7306085
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNH1 Antibody (F-5) | sc-376066 | 200 µg/ml | $322.00 | |||
RNH1 Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-540505 | 200 µg Ab; 10 µg BP | $361.00 |