RNH1 Antibody (D-3): sc-515591

RNH1 Antibody (D-3) is a mouse monoclonal IgG2b antibody that detects RNH1 in mouse and rat samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RNH1, known as the placental ribonuclease inhibitor, is a crucial 460-amino acid protein characterized by a high content of leucine and cysteine residues, which contributes to structural stability and functional versatility. RNH1 plays a vital role in regulating mRNA turnover by binding to and inhibiting a wide range of intracellular and extracellular ribonucleases, effectively keeping them in a latent form. Notably, RNH1 also interacts with angiogenin, a member of the RNase A superfamily, thereby inhibiting angiogenesis—a process essential for tumor growth and metastasis. By modulating angiogenesis, RNH1 may have significant implications in cancer gene therapy, making RNH1 a valuable target for therapeutic interventions. Anti-RNH1 antibody (D-3) is an essential tool for researchers investigating the complex roles of ribonuclease inhibitors in cellular processes and disease mechanisms.

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RNH1 Antibody (D-3) References:

1. Angiogenin activates endothelial cell phospholipase C.  |  Bicknell, R. and Vallee, BL. 1988. Proc Natl Acad Sci U S A. 85: 5961-5. PMID: 2457905
2. RNase H eliminates R-loops that disrupt DNA replication but is nonessential for efficient DSB repair.  |  Zhao, H., et al. 2018. EMBO Rep. 19: PMID: 29622660
3. FOLFOX treatment response prediction in metastatic or recurrent colorectal cancer patients via machine learning algorithms.  |  Lu, W., et al. 2020. Cancer Med. 9: 1419-1429. PMID: 31893575
4. Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.  |  Appanah, R., et al. 2020. Cell Rep. 30: 2094-2105.e9. PMID: 32075754
5. Transcription-replication conflicts as a source of common fragile site instability caused by BMI1-RNF2 deficiency.  |  Sanchez, A., et al. 2020. PLoS Genet. 16: e1008524. PMID: 32142505
6. DNA-RNA hybrids at DSBs interfere with repair by homologous recombination.  |  Ortega, P., et al. 2021. Elife. 10: PMID: 34236317
7. Dissection of paracrine/autocrine interplay in lung tumor microenvironment mimicking cancer cell-monocyte co-culture models reveals proteins that promote inflammation and metastasis.  |  Amin, A., et al. 2023. BMC Cancer. 23: 926. PMID: 37784035
8. Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification.  |  Shi, L., et al. 2024. J Tissue Eng. 15: 20417314241268912. PMID: 39301507
9. Residues 287-301 of human ribonuclease inhibitor do not affect ribonuclease activity and inhibitor binding; a reply.  |  Fominaya, J., et al. 1993. Biochim Biophys Acta. 1203: 168-70. PMID: 8218387
10. A cytotoxic ribonuclease. Study of the mechanism of onconase cytotoxicity.  |  Wu, Y., et al. 1993. J Biol Chem. 268: 10686-93. PMID: 8486718

The preferred RNH1 antibody is RNH1 Antibody (A-9): sc-365783.