Date published: 2026-4-28

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RNA pol σ N Antibody (6RN3): sc-101598

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Datasheets
  • RNA pol σ N Antibody (6RN3) is a mouse monoclonal IgG2a provided at 100 µl ascites
  • raised against RNA polymerase factor N of E. coli origin, with epitope mapping to amino acids 88-137
  • recommended for detection of RNA polymerase σ factor N of E. coli origin by WB
  • also provided azide-free for inhibition of transcription initiation in S30, sc-101598 L, 100 µl ascites
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RNA pol σ N Antibody (6RN3). This work is in progress.
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    RNA pol σ N Antibody (6RN3) is a mouse monoclonal IgG2a antibody that detects RNA polymerase σ factor N of E. coli origin by western blotting (WB). Anti-RNA pol σ N antibody (6RN3) is available as the non-conjugated form. RNA polymerase σ factor N, also referred to as rpoN, glnF, or ntrA, is a crucial 477 amino acid protein that belongs to the sigma-54 factor family in E. coli. RNA pol σ N (6RN3) monoclonal antibody plays a vital role in studying gene expression regulation, particularly in the transcription of genes involved in arginine catabolism, which is essential for bacteria′s ability to utilize arginine as a nitrogen source. Sigma factors, including RNA pol σ N, are integral to transcription initiation, as these proteins facilitate RNA polymerase binding to specific promoter regions on DNA, ensuring accurate and timely expression of genes necessary for organism survival and adaptation. RNA pol σ N mediates the expression of critical proteins, underscoring its importance in E. coli metabolic processes and making RNA pol σ N (6RN3) antibody significant for studies related to bacterial physiology and gene regulation.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    RNA pol σ N Antibody (6RN3) References:

    1. The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.  |  Buck, M., et al. 2000. J Bacteriol. 182: 4129-36. PMID: 10894718
    2. Enhancement of the synthesis of RpoN, Cra, and H-NS by polyamines at the level of translation in Escherichia coli cultured with glucose and glutamate.  |  Terui, Y., et al. 2007. J Bacteriol. 189: 2359-68. PMID: 17220219
    3. Role of eukaryotic-type functional domains found in the prokaryotic enhancer receptor factor sigma 54.  |  Sasse-Dwight, S. and Gralla, JD. 1990. Cell. 62: 945-54. PMID: 2203540
    4. Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.  |  Hunt, TP. and Magasanik, B. 1985. Proc Natl Acad Sci U S A. 82: 8453-7. PMID: 2867543
    5. Whole set of constitutive promoters for RpoN sigma factor and the regulatory role of its enhancer protein NtrC in Escherichia coli K-12.  |  Shimada, T., et al. 2021. Microb Genom. 7: PMID: 34787538
    6. RpoN Regulon in Erwinia amylovora Revealed by Transcriptional Profiling and In Silico Binding Site Analysis.  |  Yang, HW., et al. 2023. Phytopathology. 113: 183-193. PMID: 35994732
    7. Multiple regulatory inputs including cell envelope stress orchestrate expression of the Escherichia coli rpoN operon.  |  Sikora, F., et al. 2024. Mol Microbiol. 122: 11-28. PMID: 38770591
    8. Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant.  |  Powell, BS., et al. 1995. J Biol Chem. 270: 4822-39. PMID: 7876255
    9. Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli.  |  Jones, DH., et al. 1994. Microbiology (Reading). 140 (Pt 5): 1035-43. PMID: 8025669
    10. Location of the rpoN gene on the physical map of Escherichia coli.  |  Merrick, M., et al. 1993. J Bacteriol. 175: 1548-9. PMID: 8444817
    11. Physical map location of the rpoN gene of Escherichia coli.  |  Imaishi, H., et al. 1993. J Bacteriol. 175: 1550-1. PMID: 8444818
    12. Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of four species of sigma subunit under various growth conditions.  |  Jishage, M., et al. 1996. J Bacteriol. 178: 5447-51. PMID: 8808934

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    RNA pol σ N Antibody (6RN3)

    sc-101598
    100 µl ascites
    $339.00

    RNA pol σ N Antibody (6RN3) L

    sc-101598 L
    100 µl ascites
    $322.00