RNA pol σ H Antibody (3RH3): sc-101599
- RNA pol σ H Antibody (3RH3) is a mouse monoclonal IgG1 provided at 100 µl ascites
- raised against RNA polymerase factor H of E. coli origin, with epitope mapping to amino acids 1-25
- recommended for detection of RNA polymerase σ factor H of E. coli origin by WB and IP
- also provided azide-free for inhibition of transcription initiation in S-30 and in vitro, sc-101599 L, 100 µl ascites
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RNA pol σ H Antibody (3RH3). This work is in progress.
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RNA pol σ H Antibody (3RH3) is a mouse monoclonal IgG1 antibody that detects RNA polymerase σ factor H of E. coli origin by western blotting (WB) and immunoprecipitation (IP). anti-RNA pol σ H antibody (3RH3) is available as the non-conjugated form. RNA polymerase σ factor H, also referred to as rpoH, fam, hin, or htpR, is a crucial 284 amino acid protein that belongs to the sigma-70 factor family of E. coli peptides. RNA polymerase σ factor H plays a vital role in the bacterial response to stress, particularly in the regulation of heat shock proteins, which are essential for protecting cells from damage during elevated temperatures. By mediating the expression of heat shock promoters, RNA polymerase σ factor H ensures that appropriate chaperone proteins are synthesized, thereby facilitating proper protein folding and preventing aggregation under stress conditions. RNA polymerase σ factor H initiates transcription in response to environmental changes, underscoring its importance in bacterial survival and adaptation, making RNA polymerase σ factor H a key target for studies related to stress response mechanisms in prokaryotes.
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RNA pol σ H Antibody (3RH3) References:
- Regulation of the heat-shock response. | Yura, T. and Nakahigashi, K. 1999. Curr Opin Microbiol. 2: 153-8. PMID: 10322172
- Cloning and Expression of the rpoH Gene from Escherichia coli and Its Product Purification. | Chen, YH., et al. 1996. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 28: 170-176. PMID: 12237699
- Structure-function studies of Escherichia coli RpoH (sigma32) by in vitro linker insertion mutagenesis. | Narberhaus, F. and Balsiger, S. 2003. J Bacteriol. 185: 2731-8. PMID: 12700252
- Global transcriptome response of recombinant Escherichia coli to heat-shock and dual heat-shock recombinant protein induction. | Harcum, SW. and Haddadin, FT. 2006. J Ind Microbiol Biotechnol. 33: 801-14. PMID: 16680459
- Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress. | Nonaka, G., et al. 2006. Genes Dev. 20: 1776-89. PMID: 16818608
- A sigma54-dependent promoter in the regulatory region of the Escherichia coli rpoH gene. | Janaszak, A., et al. 2007. Microbiology (Reading). 153: 111-23. PMID: 17185540
- Region 2.1 of the Escherichia coli heat-shock sigma factor RpoH (sigma32) is necessary but not sufficient for degradation by the FtsH protease. | Obrist, M., et al. 2007. Microbiology (Reading). 153: 2560-2571. PMID: 17660420
- Analysis of sigma32 mutants defective in chaperone-mediated feedback control reveals unexpected complexity of the heat shock response. | Yura, T., et al. 2007. Proc Natl Acad Sci U S A. 104: 17638-43. PMID: 17968012
- Heat-induced synthesis of sigma32 in Escherichia coli: structural and functional dissection of rpoH mRNA secondary structure. | Morita, M., et al. 1999. J Bacteriol. 181: 401-10. PMID: 9882652
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNA pol σ H Antibody (3RH3) | sc-101599 | 100 µl ascites | $333.00 | |||
RNA pol σ H Antibody (3RH3) L | sc-101599 L | 100 µl ascites | $316.00 |