RING1B Antibody (N-32): sc-101109

RING1B Antibody (N-32) is a mouse monoclonal IgG2a kappa light chain antibody that detects RING1B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-RING1B antibody (N-32) is available as the non-conjugated format. RING1B, also known as E3 ubiquitin-protein ligase RING2, plays a crucial role in gene expression regulation through involvement in Polycomb group (PcG) protein complexes, which maintain silencing of developmental and cell proliferation genes. RING1B protein is primarily located in the nucleus, participating in chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) formation. RING1B protein is essential for Histone H2A monoubiquitination, a modification contributing to transcription repression during development. A RING-type zinc finger domain in RING1B enables interactions with other proteins, such as Huntington interacting protein 2 (HIP2), facilitating E3 ubiquitin ligase activity. RING1B protein is implicated in random and imprinted X chromosome inactivation in female mammals, highlighting significance in epigenetic regulation. Disrupted RING1B expression links to severe developmental defects, including axial skeletal abnormalities and gastrulation arrest in mice, underscoring vital roles in embryonic development and gene regulation.

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RING1B Antibody (N-32) References:

1. E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme.  |  Lee, SJ., et al. 2001. FEBS Lett. 503: 61-4. PMID: 11513855
2. Binding of the RING polycomb proteins to specific target genes in complex with the grainyhead-like family of developmental transcription factors.  |  Tuckfield, A., et al. 2002. Mol Cell Biol. 22: 1936-46. PMID: 11865070
3. Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice.  |  Suzuki, M., et al. 2002. Development. 129: 4171-83. PMID: 12183370
4. Rnf2 (Ring1b) deficiency causes gastrulation arrest and cell cycle inhibition.  |  Voncken, JW., et al. 2003. Proc Natl Acad Sci U S A. 100: 2468-73. PMID: 12589020
5. Role of histone H2A ubiquitination in Polycomb silencing.  |  Wang, H., et al. 2004. Nature. 431: 873-8. PMID: 15386022
6. Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation.  |  de Napoles, M., et al. 2004. Dev Cell. 7: 663-76. PMID: 15525528
7. Polycomb complexes repress developmental regulators in murine embryonic stem cells.  |  Boyer, LA., et al. 2006. Nature. 441: 349-53. PMID: 16625203
8. The polycomb protein Ring1B generates self atypical mixed ubiquitin chains required for its in vitro histone H2A ligase activity.  |  Ben-Saadon, R., et al. 2006. Mol Cell. 24: 701-711. PMID: 17157253

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RING1B Antibody (N-32) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-RING1B Antibody (N-32) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RING1B Antibody (N-32) Specifications

Alternate Product Name: RING1B

Product Clone Name: N-32

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 192-291

Epitope Target: RING1B

Epitope Species: human

Product Reactivity/Detection: RING1B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for RING1B Antibody (N-32): N/A