RIL Antibody (D-8): sc-166582

RIL Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects RIL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RIL (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RIL, or reversion-induced LIM protein, also known as PDZ and LIM domain protein 4, is a crucial 334 amino acid protein that plays a significant role in cellular signaling and development. RIL contains one PDZ (DHR) domain and one LIM zinc-binding domain, which are essential for interactions with other proteins. The LIM zinc-binding domain of RIL interacts with the second and fourth PDZ domains of Fap-1, potentially leading to RIL dephosphorylation, a process that may influence various cellular functions. RIL expression in human bone marrow stromal cells indicates involvement in osteoblast development and function, which is vital for bone health. Variations in RIL gene have been associated with an allelic dose effect on bone mineral density, a key factor in osteoporosis risk. RIL exists as two isoforms, with isoform 2 being particularly relevant in the brain, highlighting diverse roles in different tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RIL Antibody (D-8) References:

1. The zyxin-related protein TRIP6 interacts with PDZ motifs in the adaptor protein RIL and the protein tyrosine phosphatase PTP-BL.  |  Cuppen, E., et al. 2000. Eur J Cell Biol. 79: 283-93. PMID: 10826496
2. Structure, dynamics and binding characteristics of the second PDZ domain of PTP-BL.  |  Walma, T., et al. 2002. J Mol Biol. 316: 1101-10. PMID: 11884147
3. Association of genetic variation of the RIL gene, encoding a PDZ-LIM domain protein and localized in 5q31.1, with low bone mineral density in adult Japanese women.  |  Omasu, F., et al. 2003. J Hum Genet. 48: 342-5. PMID: 12908099
4. The PDZ-LIM protein RIL modulates actin stress fiber turnover and enhances the association of alpha-actinin with F-actin.  |  Vallenius, T., et al. 2004. Exp Cell Res. 293: 117-28. PMID: 14729062
5. The interaction of PTP-BL PDZ domains with RIL: an enigmatic role for the RIL LIM domain.  |  van den Berk, LC., et al. 2004. Mol Biol Rep. 31: 203-15. PMID: 15663004
6. RIL, a LIM gene on 5q31, is silenced by methylation in cancer and sensitizes cancer cells to apoptosis.  |  Boumber, YA., et al. 2007. Cancer Res. 67: 1997-2005. PMID: 17332327
7. Expression of ril, a novel LIM domain gene, is down-regulated in Hras-transformed cells and restored in phenotypic revertants.  |  Kiess, M., et al. 1995. Oncogene. 10: 61-8. PMID: 7824279
8. The human RIL gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts.  |  Bashirova, AA., et al. 1998. Gene. 210: 239-45. PMID: 9573374

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RIL Antibody (D-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RIL Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RIL Antibody (D-8) Specifications

Alternate Product Name: RIL

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 71-200

Epitope Target: RIL

Epitope Species: human

Product Reactivity/Detection: RIL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RIL Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin