RIG-I Antibody (D-12): sc-376845

RIG-I Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects RIG-I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RIG-I (D-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RIG-I, also known as DDX58, plays a crucial role in the innate immune response by recognizing viral components, particularly double-stranded RNA (dsRNA), which is a hallmark of viral replication. This 925 amino acid protein functions as an interferon-inducible DExD/H box RNA helicase, activating type I interferon (IFN) production in response to viral infections. RIG-I′s ability to detect elevated levels of dsRNA, which are typically low in uninfected cells, is vital for initiating an effective antiviral response. Upon binding to viral dsRNA through its helicase domain, RIG-I transmits an activation signal that leads to the expression of IFN via IκB kinase-related kinases. RIG-I is predominantly located in the cytoplasm of fibroblasts and conventional dendritic cells, where RIG-I plays a key role in distinguishing between various RNA viruses, thereby enhancing the host′s ability to mount a targeted immune response.

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RIG-I Antibody (D-12) References:

1. Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.  |  Sumpter, R., et al. 2005. J Virol. 79: 2689-99. PMID: 15708988
2. RIG-I: an essential regulator of virus-induced interferon production.  |  Heim, MH. 2005. J Hepatol. 42: 431-3. PMID: 15710232
3. Inhibition of RIG-I-dependent signaling to the interferon pathway during hepatitis C virus expression and restoration of signaling by IKKepsilon.  |  Breiman, A., et al. 2005. J Virol. 79: 3969-78. PMID: 15767399
4. Cell type-specific involvement of RIG-I in antiviral response.  |  Kato, H., et al. 2005. Immunity. 23: 19-28. PMID: 16039576
5. Hepatitis A virus suppresses RIG-I-mediated IRF-3 activation to block induction of beta interferon.  |  Fensterl, V., et al. 2005. J Virol. 79: 10968-77. PMID: 16103148
6. [Cell type specific involvement of RIG-I in antiviral responses].  |  Kato, H., et al. 2006. Nihon Rinsho. 64: 1244-7. PMID: 16838639
7. Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway.  |  Paz, S., et al. 2006. Cell Mol Biol (Noisy-le-grand). 52: 17-28. PMID: 16914100
8. EB virus-encoded RNAs are recognized by RIG-I and activate signaling to induce type I IFN.  |  Samanta, M., et al. 2006. EMBO J. 25: 4207-14. PMID: 16946700
9. Immune signaling by RIG-I-like receptors.  |  Loo, YM. and Gale, M. 2011. Immunity. 34: 680-92. PMID: 21616437
10. The molecular mechanism of RIG-I activation and signaling.  |  Thoresen, D., et al. 2021. Immunol Rev. 304: 154-168. PMID: 34514601
11. Exploiting RIG-I-like receptor pathway for cancer immunotherapy.  |  Jiang, Y., et al. 2023. J Hematol Oncol. 16: 8. PMID: 36755342
12. NAD+ precursor supplementation prevents mtRNA/RIG-I-dependent inflammation during kidney injury.  |  Doke, T., et al. 2023. Nat Metab. 5: 414-430. PMID: 36914909

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RIG-I Antibody (D-12) has 43 citations in a variety of scientific publications.

Primary antibodies like Anti-RIG-I Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RIG-I Antibody (D-12) Specifications

Alternate Product Name: DDX58

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 5-300

Epitope Target: RIG-I

Epitope Species: mouse

Product Reactivity/Detection: RIG-I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 43 citations in various scientific publications

Available Conjugates/Forms for RIG-I Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin