Rieske FeS Antibody (A-5): sc-271609

Rieske FeS Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rieske FeS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Rieske FeS antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rieske FeS protein plays a crucial role in the mitochondrial respiratory chain, specifically as a key component of complex III, which is essential for cellular respiration and energy production. Rieske FeS protein facilitates the transfer of electrons from ubiquinol to cytochrome c, a process vital for ATP synthesis in aerobic organisms. Proper functioning of Rieske FeS protein is critical, as any dysfunction can lead to impaired energy metabolism and has been implicated in various diseases, including certain aggressive forms of breast cancer. Rieske FeS protein may contribute to the development of more aggressive cancer phenotypes, highlighting its importance not only in cellular respiration but also in cancer biology.

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Rieske FeS Antibody (A-5) References:

1. Double minute chromosomes in acute myeloid leukemia and myelodysplastic syndrome: identification of new amplification regions by fluorescence in situ hybridization and spectral karyotyping.  |  Sait, SN., et al. 2002. Genes Chromosomes Cancer. 34: 42-7. PMID: 11921281
2. CA125 and UQCRFS1 FISH studies of ovarian carcinoma.  |  Kaneko, SJ., et al. 2003. Gynecol Oncol. 90: 29-36. PMID: 12821338
3. The Rieske FeS protein encoded and synthesized within mitochondria complements a deficiency in the nuclear gene.  |  Golik, P., et al. 2003. Proc Natl Acad Sci U S A. 100: 8844-9. PMID: 12837937
4. Rhodobacter capsulatus mutants lacking the Rieske FeS protein form a stable cytochrome bc1 subcomplex with an intact quinone reduction site.  |  Davidson, E., et al. 1992. Biochemistry. 31: 3351-8. PMID: 1313293
5. Ubiquinol cytochrome c reductase (UQCRFS1) gene amplification in primary breast cancer core biopsy samples.  |  Ohashi, Y., et al. 2004. Gynecol Oncol. 93: 54-8. PMID: 15047214
6. A Brownian dynamics study of the interactions of the luminal domains of the cytochrome b6f complex with plastocyanin and cytochrome c6: the effects of the Rieske FeS protein on the interactions.  |  Haddadian, EJ. and Gross, EL. 2006. Biophys J. 91: 2589-600. PMID: 16844750
7. The effects of Liuwei Dihuang decoction on the gene expression in the hippocampus of senescence-accelerated mouse.  |  Cheng, XR., et al. 2007. Fitoterapia. 78: 175-81. PMID: 17337329
8. Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield.  |  Simkin, AJ., et al. 2017. Plant Physiol. 175: 134-145. PMID: 28754840
9. Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster.  |  Zhou, X., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33876763
10. Structure, sequence and location of the UQCRFS1 gene for the human Rieske Fe-S protein.  |  Pennacchio, LA., et al. 1995. Gene. 155: 207-11. PMID: 7721092
11. Assignment of the gene (UQCRFS1) for the Rieske iron-sulfur protein subunit of the mitochondrial cytochrome bc1 complex to the 22q13 and 19q12-q13.1 regions of the human genome.  |  Duncan, AM., et al. 1994. Genomics. 21: 281-3. PMID: 8088805
12. Light-stimulated degradation of an unassembled Rieske FeS protein by a thylakoid-bound protease: the possible role of the FtsH protease.  |  Ostersetzer, O. and Adam, Z. 1997. Plant Cell. 9: 957-65. PMID: 9212469

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rieske FeS Antibody (A-5) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Rieske FeS Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rieske FeS Antibody (A-5) Specifications

Alternate Product Name: Rieske FeS

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 171-223

Epitope Target: Rieske FeS

Epitope Species: human

Product Reactivity/Detection: Rieske FeS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Rieske FeS Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin