RICH2 Antibody (A-7): sc-390609
- RICH2 Antibody (A-7) is a mouse monoclonal IgG1 κ RICH2 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 465-492 within an internal region of RICH2 of human origin
- RICH2 Antibody (A-7) is recommended for detection of RICH2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-RICH2 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RICH2 Antibody (A-7) for WB applications. These reagents are now offered in bundles with RICH2 Antibody (A-7) (see ordering information below).
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RICH2 Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects RICH2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RICH2 Antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RICH2, also known as RhoGAP interacting with CIP4 homologs protein 2 or ARHGAP44, is an 818 amino acid protein predominantly expressed in the brain, where RICH2 plays a crucial role in regulating cellular signaling pathways. Specifically, RICH2 functions as a GTPase activator for Rac1, Cdc42, and Rho-type GTPases by facilitating their conversion to an inactive GDP-bound state, which is essential for maintaining proper cellular functions such as cytoskeletal dynamics and cell migration. RICH2 contains a BAR domain and a Rho-GAP domain, and is encoded by a gene located on human chromosome 17p12, a region that harbors over 1,200 genes, including key tumor suppressor genes like p53 and BRCA1. The integrity of these pathways is vital, as disruptions can lead to various malignancies, underscoring the importance of RICH2 in cellular homeostasis and its potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RICH2 Antibody (A-7) References:
- p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis. | Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
- Distinct pattern of p53 phosphorylation in human tumors. | Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
- Rich, a rho GTPase-activating protein domain-containing protein involved in signaling by Cdc42 and Rac1. | Richnau, N. and Aspenström, P. 2001. J Biol Chem. 276: 35060-70. PMID: 11431473
- Three primary malignancies related to BRCA mutation successively occurring in a BRCA1 185delAG mutation carrier. | Piura, B., et al. 2001. Eur J Obstet Gynecol Reprod Biol. 97: 241-4. PMID: 11451557
- Closing in on a breast cancer gene on chromosome 17q. | Hall, JM., et al. 1992. Am J Hum Genet. 50: 1235-42. PMID: 1598904
- A CD317/tetherin-RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. | Rollason, R., et al. 2009. J Cell Biol. 184: 721-36. PMID: 19273615
- The Li-Fraumeni syndrome: an inherited susceptibility to cancer. | Evans, SC. and Lozano, G. 1997. Mol Med Today. 3: 390-5. PMID: 9302689
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RICH2 Antibody (A-7) | sc-390609 | 200 µg/ml | $316.00 | |||
RICH2 Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-530201 | 200 µg Ab; 10 µg BP | $354.00 | |||
RICH2 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-523602 | 200 µg Ab, 40 µg BP | $354.00 | |||
RICH2 Antibody (A-7): m-IgG1 BP-HRP Bundle | sc-543925 | 200 µg Ab; 20 µg BP | $354.00 | |||
RICH2 Antibody (A-7) AC | sc-390609 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RICH2 Antibody (A-7) HRP | sc-390609 HRP | 200 µg/ml | $316.00 | |||
RICH2 Antibody (A-7) FITC | sc-390609 FITC | 200 µg/ml | $330.00 | |||
RICH2 Antibody (A-7) PE | sc-390609 PE | 200 µg/ml | $343.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 488 | sc-390609 AF488 | 200 µg/ml | $357.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 546 | sc-390609 AF546 | 200 µg/ml | $357.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 594 | sc-390609 AF594 | 200 µg/ml | $357.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 647 | sc-390609 AF647 | 200 µg/ml | $357.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 680 | sc-390609 AF680 | 200 µg/ml | $357.00 | |||
RICH2 Antibody (A-7) Alexa Fluor® 790 | sc-390609 AF790 | 200 µg/ml | $357.00 | |||
RICH2 (A-7) Neutralizing Peptide | sc-390609 P | 100 µg/0.5 ml | $68.00 |