RIC-3 Antibody (G-8): sc-377408

RIC-3 Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects RIC-3 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RIC-3 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RIC-3, known as resistant to inhibitors of cholinesterase-3, serves as the mammalian homolog of the ric-3 protein found in Caenorhabditis elegans. RIC-3 is characterized by two transmembrane domains and a coiled-coil domain, and predominantly expresses in neurons, where RIC-3 localizes to the endoplasmic reticulum. This localization is crucial as RIC-3 plays a significant role in the folding and assembly of nicotinic acetylcholine receptors (nAChRs), enhancing the stability and surface expression of various nAChR subtypes and some 5-HT3 receptors. RIC-3′s ability to facilitate proper receptor assembly underscores its importance as a regulator of receptor expression, which is vital for neurotransmission and synaptic plasticity. Multiple isoforms of RIC-3 exist, each exhibiting distinct localization patterns and potentially varying effects on receptor expression, further highlighting RIC-3′s complexity and significance in neuronal function.

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RIC-3 Antibody (G-8) References:

1. Conservation within the RIC-3 gene family. Effectors of mammalian nicotinic acetylcholine receptor expression.  |  Halevi, S., et al. 2003. J Biol Chem. 278: 34411-7. PMID: 12821669
2. Ric-3 promotes functional expression of the nicotinic acetylcholine receptor alpha7 subunit in mammalian cells.  |  Williams, ME., et al. 2005. J Biol Chem. 280: 1257-63. PMID: 15504725
3. Cell surface expression of 5-hydroxytryptamine type 3 receptors is promoted by RIC-3.  |  Cheng, A., et al. 2005. J Biol Chem. 280: 22502-7. PMID: 15809299
4. Dual role of the RIC-3 protein in trafficking of serotonin and nicotinic acetylcholine receptors.  |  Castillo, M., et al. 2005. J Biol Chem. 280: 27062-8. PMID: 15927954
5. RIC-3 affects properties and quantity of nicotinic acetylcholine receptors via a mechanism that does not require the coiled-coil domains.  |  Ben-Ami, HC., et al. 2005. J Biol Chem. 280: 28053-60. PMID: 15932871
6. RIC-3 enhances functional expression of multiple nicotinic acetylcholine receptor subtypes in mammalian cells.  |  Lansdell, SJ., et al. 2005. Mol Pharmacol. 68: 1431-8. PMID: 16120769
7. Cytoplasmic regions adjacent to the M3 and M4 transmembrane segments influence expression and function of alpha7 nicotinic acetylcholine receptors. A study with single amino acid mutants.  |  Castelán, F., et al. 2007. J Neurochem. 100: 406-15. PMID: 17076762
8. Differential subcellular localization of RIC-3 isoforms and their role in determining 5-HT3 receptor composition.  |  Cheng, A., et al. 2007. J Biol Chem. 282: 26158-66. PMID: 17609200
9. Lack of RIC-3 congruence with beta2 subunit-containing nicotinic acetylcholine receptors in bipolar disorder.  |  Severance, EG. and Yolken, RH. 2007. Neuroscience. 148: 454-60. PMID: 17640815
10. RIC-3: a nicotinic acetylcholine receptor chaperone.  |  Millar, NS. 2008. Br J Pharmacol. 153 Suppl 1: S177-83. PMID: 18246096
11. Regulation of RIC-3 and of nAChR expression.  |  Ben-David, Y. and Treinin, M. 2017. Oncotarget. 8: 5662-5663. PMID: 28086205
12. Speculation on How RIC-3 and Other Chaperones Facilitate α7 Nicotinic Receptor Folding and Assembly.  |  Loring, RH. 2022. Molecules. 27: PMID: 35889400

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RIC-3 Antibody (G-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RIC-3 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RIC-3 Antibody (G-8) Specifications

Alternate Product Name: RIC-3

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 88-369

Epitope Target: RIC-3

Epitope Species: human

Product Reactivity/Detection: RIC-3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RIC-3 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin