Date published: 2026-4-5

1-800-457-3801

SCBT Portrait Logo
Seach Input

Ribosomal Protein S6 Antibody (H-4): sc-74576

4.8(6)
Write a reviewAsk a question

Datasheets
  • Ribosomal Protein S6 Antibody (H-4) is a mouse monoclonal IgG1 κ, cited in 10 publications, provided at 200 µg/ml
  • raised against the N-terminus of Ribosomal Protein S6 of human origin
  • recommended for detection of 40S Ribosomal Protein S6 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Ribosomal Protein S6 Antibody (H-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Ribosomal Protein S6 Antibody (H-4) (see ordering information below).
Gene Editing Promo Banner

QUICK LINKS

SEE ALSO...

Ribosomal Protein S6 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Ribosomal Protein S6 (RPS6), also known as S6, a vital component of the 40S ribosomal subunit located in the cytoplasm where RPS6 plays a crucial role in protein synthesis. Anti-RPS6 antibody (H-4) targets the N-terminal epitope of human RPS6, ensuring precise recognition and binding. RPS6 is essential for the regulation of cell growth and proliferation, as RPS6 is a major substrate for S6 kinases that phosphorylate RPS6 in response to various extracellular signals, thereby controlling protein translation and influencing cellular function and metabolism. RPS6 monoclonal antibody (H-4) exhibits broad species reactivity, effectively recognizing RPS6 from mouse, rat, and human origins across multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Additionally, RPS6 (H-4) antibody is available in both non-conjugated form and various conjugated forms such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for diverse experimental needs. The high conservation of RPS6 across eukaryotic species underscores RPS6′s fundamental role in ribosomal function and cellular biology, making RPS6 (H-4) monoclonal antibody an invaluable tool for research in areas such as cell signaling, growth regulation, and disease mechanisms. By targeting a key protein involved in the core machinery of protein synthesis, anti-RPS6 antibody (H-4) facilitates advanced studies into cellular processes and therapeutic developments.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Ribosomal Protein S6 Antibody (H-4)

sc-74576
200 µg/ml
$322.00

Ribosomal Protein S6 Antibody (H-4): m-IgG Fc BP-HRP Bundle

sc-539551
200 µg Ab; 10 µg BP
$361.00

Ribosomal Protein S6 Antibody (H-4): m-IgG1 BP-HRP Bundle

sc-541552
200 µg Ab; 20 µg BP
$361.00

I am using Ribosomal Protein S6 (H-4): sc-74576 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_3, d_26, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_74576, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works with yeast S. cerevisaeThis antibody has good sensitivity and specificity towards yeast S. cerevisae Rps6. Yeast lysates were collected using the culture boiling technique resuspending in 1x loading buffer (30 uL/1.0 OD600*mL cells collected) and loading 5 uL per well (https://doi.org/10.1002/yea.1628). Performed in quadruplicate. Total protein staining by R-Prob Sigma kit to show consistent protein levels per lane. Antibody used at 1:1000 dilution.
Date published: 2021-07-28
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-04-10
Rated 4 out of 5 by from No commentNo comment
Date published: 2015-03-25
Rated 5 out of 5 by from Very robust signal in WB in mouse RibosomalVery robust signal in WB in mouse Ribosomal Protein S6 transfected 293T and K-562 whole cell lysates. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Robust band seen in WB in Hep G2 and NIH/3T3Robust band seen in WB in Hep G2 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Very strong band seen in WB in HeLaVery strong band seen in WB in HeLa, K-562 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-03-06
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_74576, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
Ribosomal Protein S6 Antibody (H-4) is rated 4.8 out of 5 by 6.
  • y_2026, m_3, d_26, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_74576, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT