Ribosomal Protein S2 Antibody (80-H): sc-130399

Ribosomal Protein S2 Antibody (80-H) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ribosomal Protein S2 (RPS2) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RPS2 antibody (80-H) is available as the non-conjugated form. RPS2 plays a crucial role in the assembly and function of the ribosome, specifically as part of the 40S subunit, where RPS2 is involved in mediating aminoacyl-transfer RNA binding to the ribosome, which is essential for accurate mRNA translation. This function is particularly important as RPS2 directly influences the fidelity of protein synthesis, a process vital for cellular function and growth. Additionally, RPS2 is subject to post-translational modifications, including methylation by protein arginine methyltransferase 3 (PRMT3), which may inhibit degradation via ubiquitin-mediated proteolysis, thereby stabilizing RPS2 presence in the cell. Elevated expression levels of RPS2 have been associated with various cancers, including head and neck squamous cell carcinomas and breast and colon cancers, highlighting RPS2′s potential as a diagnostic marker for certain tumors. Nuclear export of ribosomal subunits, including RPS2, is a highly regulated process, emphasizing RPS2′s importance in maintaining cellular homeostasis and potential implications in cancer biology.

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Ribosomal Protein S2 Antibody (80-H) References:

1. Increased expression of ribosomal protein S2 in liver tumors, posthepactomized livers, and proliferating hepatocytes in vitro.  |  Kowalczyk, P., et al. 2002. Acta Biochim Pol. 49: 615-24. PMID: 12422231
2. PRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits.  |  Bachand, F. and Silver, PA. 2004. EMBO J. 23: 2641-50. PMID: 15175657
3. Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3).  |  Swiercz, R., et al. 2005. Biochem J. 386: 85-91. PMID: 15473865
4. Elevated expression of the ribosomal protein S2 gene in human tumors.  |  Chiao, PJ., et al. 1992. Mol Carcinog. 5: 219-31. PMID: 1586449
5. PRMT3 inhibits ubiquitination of ribosomal protein S2 and together forms an active enzyme complex.  |  Choi, S., et al. 2008. Biochim Biophys Acta. 1780: 1062-9. PMID: 18573314
6. Primary structure of rat ribosomal protein S2. A ribosomal protein with arginine-glycine tandem repeats and RGGF motifs that are associated with nucleolar localization and binding to ribonucleic acids.  |  Suzuki, K., et al. 1991. J Biol Chem. 266: 20007-10. PMID: 1939063
7. Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.  |  Vladimirov, SN., et al. 1996. Eur J Biochem. 239: 144-9. PMID: 8706699
8. A map of 75 human ribosomal protein genes.  |  Kenmochi, N., et al. 1998. Genome Res. 8: 509-23. PMID: 9582194

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ribosomal Protein S2 Antibody (80-H) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Ribosomal Protein S2 Antibody (80-H) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ribosomal Protein S2 Antibody (80-H) Specifications

Alternate Product Name: Ribosomal Protein S2

Product Clone Name: 80-H

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 198-294

Epitope Target: Ribosomal Protein S2

Epitope Species: human

Product Reactivity/Detection: Ribosomal Protein S2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Ribosomal Protein S2 Antibody (80-H): N/A