Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ribosomal Protein S13 CRISPR/Cas9 KO Plasmid (h): sc-406367
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein S13 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ribosomal Protein S13 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ribosomal Protein S13 Antibody (C-3): sc-398690
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein S13 CRISPR/Cas9 KO Plasmid (h) | sc-406367 | 20 µg | $397.00 |
Overview
RPS13 encodes ribosomal protein S13, an essential component of the 40S small ribosomal subunit that supports ribosome assembly, rRNA processing, and accurate mRNA decoding during translation. Through its role in cytosolic protein synthesis, RPS13 contributes to cellular growth control and proteostasis and interfaces with pathways sensitive to translational capacity, including stress-adaptive and nucleolar surveillance responses. Disruption of ribosomal protein homeostasis is linked to ribosomopathies and has been associated with altered proliferation programs observed in multiple cancer contexts, making RPS13 a useful node for studying how translational output shapes cell state. As a conserved ribosomal factor, RPS13 is also relevant for investigating global translation dynamics, selective mRNA translation, and consequences of ribosome biogenesis imbalance.
Ribosomal Protein S13 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RPS13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RPS13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RPS13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein S13 protein expression.
This CRISPR knockout system enables efficient generation of RPS13-deficient cell models for investigation of Ribosomal Protein S13 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RPS13 exon(s) critical for Ribosomal Protein S13 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RPS13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ribosomal Protein S13 CRISPR/Cas9 KO Plasmid (h) and Ribosomal Protein S13 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RPS13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ribosomal Protein S13 HDR Plasmid (h) and Ribosomal Protein S13 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RPS13 homology arms to support homology-directed repair at defined RPS13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.