Date published: 2026-4-12
Visit Santa Cruz Animal Health

1-800-457-3801

SCBT Portrait Logo
Seach Input
Contact Us
Sign In Account
Quick Order
Cart (0)
Ribosomal Protein L11 Antibody (2A1) - Immunohistochemistry - Image 346122
Ribosomal Protein L11 Antibody (2A1): sc-293224. Immunofluor-escence staining of methanol-fixed HeLa cells showing nuclear localization.
Ribosomal Protein L11 Antibody (2A1) - Immunohistochemistry - Image 346122
Ribosomal Protein L11 Antibody (2A1): sc-293224. Immunofluor-escence staining of methanol-fixed HeLa cells showing nuclear localization.
Ribosomal Protein L11 Antibody (2A1) - Western Blotting - Image 348824
Ribosomal Protein L11 Antibody (2A1): sc-293224. Western blot analysis of human recombinant Ribosomal Protein L11 fusion protein.
Ribosomal Protein L11 Antibody (2A1): sc-293224. Immunofluor-escence staining of methanol-fixed HeLa cells showing nuclear localization.

Ribosomal Protein L11 Antibody (2A1): sc-293224

5.0(1)
Write a reviewAsk a question

Datasheets
  • Ribosomal Protein L11 Antibody (2A1) is a mouse monoclonal IgG2b κ Ribosomal Protein L11 antibody provided at 100 µg/ml
  • raised against amino acids 1-177 representing full length Ribosomal Protein L11 of human origin
  • recommended for detection of Ribosomal Protein L11 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ribosomal Protein L11 Antibody (2A1). This work is in progress.
    Gene Editing Promo Banner

    QUICK LINKS

    Support Products
    Description
    Gene Info
    Datasheets and Protocols

    SEE ALSO...

    Additional Ribosomal Protein Antibodies including Ribosomal Protein L13, Ribosomal Protein L14, Ribosomal Protein L17, Ribosomal Protein L18, Ribosomal Protein L19, Ribosomal Protein L21, Ribosomal Protein L22, Ribosomal Protein L23a, Ribosomal Protein L30, Ribosomal Protein L36a, Ribosomal Protein L39L, Ribosomal Protein L4, Ribosomal Protein L9, Ribosomal Protein LP0, Ribosomal Protein S10 and Ribosomal Protein S13

    Ribosomal Protein L11 Antibody (2A1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ribosomal Protein L11 (RPL11) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-RPL11 antibody (2A1) is available as a non-conjugated format. RPL11 plays a crucial role in ribosome assembly and function, essential for protein synthesis in all living cells. RPL11 (2A1) monoclonal antibody localizes to the nucleolus, where RPL11 participates in ribosomal RNA processing and maturation, vital for ribosome formation. RPL11 function remains critical since disruption in ribosome biogenesis can lead to various diseases, including cancer and ribosomopathies. The gene encoding human RPL11 is located on chromosome 1p36.1-p35, and evolutionary conservation across species underscores RPL11′s fundamental role in cellular biology. RPL11 (2A1) antibody serves as an invaluable tool for researchers studying ribosome function and related cellular processes.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ribosomal Protein L11 Antibody (2A1) References:

    1. Assignment of the L11 ribosomal protein gene (RPL11) to human chromosome 1p36.1-->p35 by in situ hybridization.  |  Graphodatsky, AS., et al. 1999. Cytogenet Cell Genet. 84: 97-8. PMID: 10343117
    2. Ribosomal protein L11 negatively regulates oncoprotein MDM2 and mediates a p53-dependent ribosomal-stress checkpoint pathway.  |  Zhang, Y., et al. 2003. Mol Cell Biol. 23: 8902-12. PMID: 14612427
    3. The mapping of seven intron-containing ribosomal protein genes shows they are unlinked in the human genome.  |  Feo, S., et al. 1992. Genomics. 13: 201-7. PMID: 1577483
    4. The primary structure of rat ribosomal proteins: the amino acid sequences of L27a and L28 and corrections in the sequences of S4 and S12.  |  Wool, IG., et al. 1990. Biochim Biophys Acta. 1050: 69-73. PMID: 2207170
    5. Physical and functional interaction between ribosomal protein L11 and the tumor suppressor ARF.  |  Dai, MS., et al. 2012. J Biol Chem. 287: 17120-17129. PMID: 22467867
    6. Proapoptotic Requirement of Ribosomal Protein L11 in Ribosomal Stress-Challenged Cortical Neurons.  |  Slomnicki, LP., et al. 2018. Mol Neurobiol. 55: 538-553. PMID: 27975169
    7. Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe.  |  Gross, T., et al. 1988. Curr Genet. 13: 57-63. PMID: 2834104
    8. Ribosomal Protein L11 Selectively Stabilizes a Tertiary Structure of the GTPase Center rRNA Domain.  |  Welty, R., et al. 2020. J Mol Biol. 432: 991-1007. PMID: 31874150
    9. The primary structure of the human ribosomal protein S6 derived from a cloned cDNA.  |  Heinze, H., et al. 1988. J Biol Chem. 263: 4139-44. PMID: 3279029
    10. Nucleolar Stress Response via Ribosomal Protein L11 Regulates Topoisomerase Inhibitor Sensitivity of P53-Intact Cancers.  |  Ishihara, Y., et al. 2022. Int J Mol Sci. 23: PMID: 36555627
    11. Regulation of bacterial stringent response by an evolutionarily conserved ribosomal protein L11 methylation.  |  Walukiewicz, HE., et al. 2024. mBio. 15: e0177324. PMID: 39189746
    12. Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs.  |  Frigerio, JM., et al. 1995. Biochim Biophys Acta. 1262: 64-8. PMID: 7772601

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Ribosomal Protein L11 Antibody (2A1)

    sc-293224
    100 µg/ml
    		$322.00

    Why is your antibody recognising a 40 kDa protein - RPL11 is about 20 kDa. It should also be predominantly cytoplasmic - as that is where the ribosomes are. My lab works on ribosome biogenesis and I think this antibody should be removed from your stock

    Asked by: NickWatkins
    Thank you very much for the question! The full-length Ribosomal Protein L11 of human origin has 177 amino acids. The calculated molecular weight of this protein to be detected by standard WB techniques is expected to be around 20kDa. This is also the weight we refer to on the corresponding product datasheet you can download by clicking the following link: https://datasheets.scbt.com/sc-293224.pdf The WB image we show on this product page is showing a Ribosomal Protein L11 - GST fusion protein with the corresponding higher molecular weight. This is the correct mw size we expected in this control experiment but of course not the size to be detected on endogenously expressed Ribosomal Protein L11. In the IF staining of methanol-fixed HeLa cells our labs have performed this antibody was showing nuclear localization. According to the protein literature we found this protein should be expressed predominantly in the cytoplasma but we also found supporting information about IF localization in the nucleus. Our labs will look further into this and post additional data here on the product page upon availability.
    Answered by: Technical Support Europe
    Date published: 2017-09-18

    Why is the RPL11 signal in the nucleus when RPL11 is predominantly a cytoplasmic protein? This suggests to me that the antibody is non-functional

    Asked by: NickWatkins
    Thank you for your question. HPRD and UniProt list nuclear and nucleolar as subcellular localizations for RP11; links to their websites are provided below for reference. However, we will also ask our lab to reevaluate the data based on your feedback. If we are able to provide any new or revised data, it will be updated on our website. http://www.uniprot.org/uniprot/P62913#subcellular_location http://www.hprd.org/summary?hprd_id=10364&isoform_id=10364_1&isoform_name=Isoform_1
    Answered by: Technical Support
    Date published: 2017-08-01

    Can sc-293224: Ribosomal Protein L11 (2A1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Professor Griffin
    Thank you for your question. Yes, sc-293224: Ribosomal Protein L11 (2A1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
    • y_2026, m_4, d_9, h_7CST
    • bvseo_bulk, prod_bvqa, vn_bulk_3.0.43
    • cp_1, bvpage1
    • co_hasquestionsanswers, tq_3
    • loc_en_US, sid_293224, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getContent, 106ms
    • QUESTIONS, PRODUCT
    Rated 5 out of 5 by from Good for Western BlotAntibody detects human recombinant Ribosomal Protein L11 fusion protein by Western Blot. -SCBT QC
    Date published: 2023-09-14
    • y_2026, m_4, d_9, h_7
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_en_US, sid_293224, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getReviews, 16ms
    • REVIEWS, PRODUCT
    Ribosomal Protein L11 Antibody (2A1) is rated 5.0 out of 5 by 1.
    • y_2026, m_4, d_9, h_7
    • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
    • cp_1, bvpage1
    • co_hasreviews, tv_0, tr_1
    • loc_en_US, sid_293224, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
    • clientName_scbt
    • bvseo_sdk, java_sdk, bvseo-4.0.0
    • CLOUD, getAggregateRating, 101ms
    • REVIEWS, PRODUCT
    1-800-457-3801
    Visit Santa Cruz Animal Health|Visit San Juan Ranch
    Santa Cruz Animal Health is a leading provider of animal health care products. ©2026 Santa Cruz Biotechnology Inc. All Rights Reserved.
    Toll-Free: 1-800-457-3801 - Terms & Conditions | Privacy Policy | Legal
    “Santa Cruz Animal Health”, “San Juan Ranch”, the San Juan Ranch logo, “UltraCruz”, “Supplement of Champions”, are registered trademarks of Santa Cruz Biotechnology, Inc. All other trademarks are the property of their respective owners.