Ribophorin I Antibody (E-7): sc-48367

Ribophorin I Antibody (E-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ribophorin I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Ribophorin I antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ribophorin I plays a crucial role in the endoplasmic reticulum (ER) as an integral membrane glycoprotein, specifically localized to the rough ER, where Ribophorin I is involved in the process of N-glycosylation of newly synthesized polypeptides. Ribophorin I is part of the oligosaccharyltransferase (OST) complex, which is essential for the proper folding and maturation of glycoproteins, thereby influencing various cellular functions and signaling pathways. Ribophorin I and II interact with other components of the OST complex, such as OST48 and DAD1, to facilitate the cotranslational addition of oligosaccharides to nascent polypeptides, a critical step in protein maturation that affects protein stability, localization, and function. Understanding Ribophorin I′s role in these processes is vital for elucidating the mechanisms of protein synthesis and modification within the cell, which has implications for various biological functions and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ribophorin I Antibody (E-7) References:

1. Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum.  |  Fu, J. and Kreibich, G. 2000. J Biol Chem. 275: 3984-90. PMID: 10660554
2. Localization of ribophorin II to the endoplasmic reticulum involves both its transmembrane and cytoplasmic domains.  |  Fu, J., et al. 2000. Eur J Cell Biol. 79: 219-28. PMID: 10826490
3. The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1.  |  Silberstein, S., et al. 1992. J Biol Chem. 267: 23658-63. PMID: 1429707
4. Ribophorin I associates with a subset of membrane proteins after their integration at the sec61 translocon.  |  Wilson, CM., et al. 2005. J Biol Chem. 280: 4195-206. PMID: 15556939
5. Ribophorin I regulates substrate delivery to the oligosaccharyltransferase core.  |  Wilson, CM., et al. 2008. Proc Natl Acad Sci U S A. 105: 9534-9. PMID: 18607003
6. Malectin forms a complex with ribophorin I for enhanced association with misfolded glycoproteins.  |  Qin, SY., et al. 2012. J Biol Chem. 287: 38080-9. PMID: 22988243
7. Association of malectin with ribophorin I is crucial for attenuation of misfolded glycoprotein secretion.  |  Takeda, K., et al. 2014. Biochem Biophys Res Commun. 454: 436-40. PMID: 25451265
8. Subcellular distribution of endogenous malectin under rest and stress conditions is regulated by ribophorin I.  |  Yang, QP., et al. 2018. Glycobiology. 28: 374-381. PMID: 29618064
9. Cell surface expression of Ribophorin I, an endoplasmic reticulum protein, over different cell types.  |  Ahmed, MZ. and Alqahtani, AS. 2024. Int J Biol Macromol. 264: 130278. PMID: 38373565
10. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase.  |  Kelleher, DJ. and Gilmore, R. 1997. Proc Natl Acad Sci U S A. 94: 4994-9. PMID: 9144178
11. Interactions among subunits of the oligosaccharyltransferase complex.  |  Fu, J., et al. 1997. J Biol Chem. 272: 29687-92. PMID: 9368036
12. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex.  |  Sanjay, A., et al. 1998. J Biol Chem. 273: 26094-9. PMID: 9748289

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ribophorin I Antibody (E-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Ribophorin I Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ribophorin I Antibody (E-7) Specifications

Alternate Product Name: RPN1

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 558-607

Epitope Target: Ribophorin I

Epitope Species: human

Product Reactivity/Detection: Ribophorin I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Ribophorin I Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin