Rho V Antibody (F-2): sc-515072

Rho V Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rho V protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rho V (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rho V, also known as ras homolog gene family, member V, is a 236 amino acid protein that plays a crucial role in regulating the actin cytoskeleton through the activation of the c-Jun N-terminal kinase (JNK) pathway. Rho V is primarily located at the cytoplasmic side of the cell membrane, functioning as a lipid anchor, and is expressed in key tissues such as the placenta, pancreas, and fetal brain. Proper functioning of Rho V is vital for maintaining cellular architecture and signaling, as Rho V is involved in processes such as cytoskeletal rearrangement, nuclear signaling, and cell growth. Dysregulation of Rho V has been implicated in various cellular transformation processes, and Rho V is encoded by a gene located on human chromosome 15, which is associated with several genetic disorders, including Angelman syndrome and Prader-Willi syndrome. Anti-Rho V antibody (F-2) effectively detects this important protein, making Rho V (F-2) monoclonal antibody a valuable tool for researchers studying the underlying mechanisms of these conditions and the broader implications of Rho GTPases in cellular function.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Rho V Antibody (F-2) References:

1. The Rho GTPase family: a Racs to Wrchs story.  |  Wherlock, M. and Mellor, H. 2002. J Cell Sci. 115: 239-40. PMID: 11839775
2. Molecular cloning and characterization of WRCH2 on human chromosome 15q15.  |  Katoh, M. 2002. Int J Oncol. 20: 977-82. PMID: 11956592
3. Critical and distinct roles of amino- and carboxyl-terminal sequences in regulation of the biological activity of the Chp atypical Rho GTPase.  |  Chenette, EJ., et al. 2005. J Biol Chem. 280: 13784-92. PMID: 15664990
4. Evolution of the Rho family of ras-like GTPases in eukaryotes.  |  Boureux, A., et al. 2007. Mol Biol Evol. 24: 203-16. PMID: 17035353
5. Autophosphorylation-dependent degradation of Pak1, triggered by the Rho-family GTPase, Chp.  |  Weisz Hubsman, M., et al. 2007. Biochem J. 404: 487-97. PMID: 17355222
6. Tyrosinemia type 1 and Angelman syndrome due to paternal uniparental isodisomy 15.  |  Ferrer-Bolufer, I., et al. 2009. J Inherit Metab Dis. 32 Suppl 1: S349-53. PMID: 20033293
7. Neuropsychiatric aspects of adult-onset Tay-Sachs disease: two case reports with several new findings.  |  Hurowitz, GI., et al. 1993. J Neuropsychiatry Clin Neurosci. 5: 30-6. PMID: 8428133
8. Chp, a homologue of the GTPase Cdc42Hs, activates the JNK pathway and is implicated in reorganizing the actin cytoskeleton.  |  Aronheim, A., et al. 1998. Curr Biol. 8: 1125-8. PMID: 9778532

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rho V Antibody (F-2) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Rho V Antibody (F-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rho V Antibody (F-2) Specifications

Alternate Product Name: Rho V

Product Clone Name: F-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 149-187

Epitope Target: Rho V

Epitope Species: human

Product Reactivity/Detection: Rho V

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Rho V Antibody (F-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin