Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rho G CRISPR/Cas9 KO Plasmid (h): sc-402608
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rho G CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rho G genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rho G Antibody (1F3 B3 E5): sc-80015
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rho G CRISPR/Cas9 KO Plasmid (h) | sc-402608 | 20 µg | $397.00 |
Overview
RHOG encodes Rho G, a member of the Rho family of small GTPases that functions as a molecular switch linking receptor signaling to actin cytoskeleton remodeling. Rho G regulates membrane ruffling, cell spreading, and migration by coordinating pathways that converge on Rac1 and Cdc42 activity and by supporting integrin-dependent adhesion dynamics. Through these processes, RHOG influences endocytic trafficking and immune cell functions such as phagocytosis and antigen-driven responses. Dysregulated Rho G signaling has been investigated in contexts where altered cytoskeletal control contributes to aberrant cell motility and inflammatory phenotypes, making it relevant for mechanistic studies of cancer cell invasion and immune cell behavior.
Rho G CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RHOG gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RHOG together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RHOG open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rho G protein expression.
This CRISPR knockout system enables efficient generation of RHOG-deficient cell models for investigation of Rho G signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RHOG exon(s) critical for Rho G function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RHOG genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rho G CRISPR/Cas9 KO Plasmid (h) and Rho G CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RHOG locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rho G HDR Plasmid (h) and Rho G HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RHOG homology arms to support homology-directed repair at defined RHOG target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.