Rheb Antibody (B-12): sc-271509

Rheb Antibody (B-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Rheb protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rheb antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rheb protein plays a crucial role in cellular signaling pathways, particularly in the regulation of mTOR (mechanistic target of rapamycin), which is essential for cell growth, proliferation, and survival. Through mTOR activation, Rheb influences various cellular processes, including protein synthesis and autophagy, making Rheb a significant player in cancer biology and metabolic disorders. Dysregulation of Rheb and associated signaling pathways can lead to uncontrolled cell growth and tumorigenesis, highlighting Rheb′s importance in cancer research. Rheb also interacts with other proteins in the Ras family, such as Raf-1, which further underscores Rheb′s role in critical signaling cascades. Understanding Rheb function and interactions remains vital for developing targeted therapies in oncology and metabolic diseases.

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Rheb Antibody (B-12) References:

1. Recent progress in the study of the Rheb family GTPases.  |  Heard, JJ., et al. 2014. Cell Signal. 26: 1950-7. PMID: 24863881
2. ras oncogenes in human cancer: a review.  |  Bos, JL. 1989. Cancer Res. 49: 4682-9. PMID: 2547513
3. Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes.  |  Lowe, DG., et al. 1987. Cell. 48: 137-46. PMID: 3098437
4. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
5. Heterologous expression and characterization of the human R-ras gene product.  |  Lowe, DG. and Goeddel, DV. 1987. Mol Cell Biol. 7: 2845-56. PMID: 3313005
6. Therapeutic Potential of AAV1-Rheb(S16H) Transduction against Neurodegenerative Diseases.  |  Nam, Y., et al. 2021. Int J Mol Sci. 22: PMID: 33802760
7. Rheb regulates nuclear mTORC1 activity independent of farnesylation.  |  Zhong, Y., et al. 2022. Cell Chem Biol. 29: 1037-1045.e4. PMID: 35294906
8. CREB3L4 promotes hepatocellular carcinoma progression and decreases sorafenib chemosensitivity by promoting RHEB-mTORC1 signaling pathway.  |  Jiang, Z., et al. 2024. iScience. 27: 108843. PMID: 38303702
9. Regulation of Raf-1 and Raf-1 mutants by Ras-dependent and Ras-independent mechanisms in vitro.  |  Dent, P., et al. 1995. Mol Cell Biol. 15: 4125-35. PMID: 7623807
10. R-Ras induces malignant, but not morphologic, transformation of NIH3T3 cells.  |  Cox, AD., et al. 1994. Oncogene. 9: 3281-8. PMID: 7936652
11. Oncogenic activation of human R-ras by point mutations analogous to those of prototype H-ras oncogenes.  |  Saez, R., et al. 1994. Oncogene. 9: 2977-82. PMID: 8084601

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rheb Antibody (B-12) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Rheb Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rheb Antibody (B-12) Specifications

Alternate Product Name: Rheb

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 41-110

Epitope Target: Rheb

Epitope Species: human

Product Reactivity/Detection: Rheb

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Rheb Antibody (B-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin