Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RGS8 CRISPR/Cas9 KO Plasmid (h): sc-403199
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RGS8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RGS8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RGS8 Antibody (F-3): sc-398949
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGS8 CRISPR/Cas9 KO Plasmid (h) | sc-403199 | 20 µg | $397.00 |
Overview
RGS8 (regulator of G protein signaling 8) is a GTPase-activating protein that accelerates termination of GPCR-initiated signaling by promoting Gα subunit inactivation. In human cells, RGS8 modulates second-messenger outputs such as cAMP/PKA and PLCβ/Ca2+ pathways, shaping the amplitude and duration of receptor-driven responses. It is enriched in neuronal contexts and contributes to control of synaptic signaling and network excitability through regulation of Gi/o- and Gq-coupled cascades. Altered RGS8 expression or activity has been investigated in relation to dysregulated neurotransmission and neuropsychiatric phenotypes, making it relevant for mechanistic studies of GPCR pathway tuning.
RGS8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RGS8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RGS8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RGS8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RGS8 protein expression.
This CRISPR knockout system enables efficient generation of RGS8-deficient cell models for investigation of RGS8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RGS8 exon(s) critical for RGS8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RGS8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RGS8 CRISPR/Cas9 KO Plasmid (h) and RGS8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RGS8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RGS8 HDR Plasmid (h) and RGS8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RGS8 homology arms to support homology-directed repair at defined RGS8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.