RGS6/7 Antibody (F-10): sc-271643
- RGS6/7 Antibody (F-10) is a mouse monoclonal IgG1 κ RGS6/7 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 306-495 deletion 454-471 (deletion 454-471) mapping at the C-terminus of RGS7 of human origin
- RGS6/7 Antibody (F-10) is recommended for detection of RGS6 and isoforms 1-4 of RGS7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RGS6/7 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RGS6/7 Antibody (F-10) for WB applications. These reagents are now offered in bundles with RGS6/7 Antibody (F-10) (see ordering information below).
RGS6/7 Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects RGS6/7 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RGS6/7 (F-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGS6/7 protein plays a crucial role in regulating G protein signaling pathways, which are essential for transmitting signals from cell surface receptors to intracellular effectors. RGS6 and RGS7 are part of the RGS family of proteins that act as GTPase-activating proteins (GAPs), enhancing GTP hydrolysis to GDP on Gα subunits, thereby terminating the signaling cascade. This regulation is vital for maintaining cellular homeostasis and ensuring signaling pathways are activated and deactivated at appropriate times. RGS6/7 protein is predominantly localized in the cytosol and forms complexes with Gβ5 subunits, which preferentially target the Gαo subunit for hydrolysis, thus inhibiting Gβ1γ2-mediated activation of phospholipase C. This intricate interplay of protein interactions and regulatory mechanisms underscores RGS6/7′s importance in modulating G protein signaling, making anti-RGS6/7 antibody (F-10) an invaluable tool for researchers studying these critical pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RGS6/7 Antibody (F-10) References:
- Fidelity of G protein beta-subunit association by the G protein gamma-subunit-like domains of RGS6, RGS7, and RGS11. | Snow, BE., et al. 1999. Proc Natl Acad Sci U S A. 96: 6489-94. PMID: 10339615
- A G-protein signaling network mediated by an RGS protein. | Guan, KL. and Han, M. 1999. Genes Dev. 13: 1763-7. PMID: 10421628
- Emerging roles for RGS proteins in cell signalling. | Hepler, JR. 1999. Trends Pharmacol Sci. 20: 376-82. PMID: 10462761
- Regulators of G protein signaling 6 and 7. Purification of complexes with gbeta5 and assessment of their effects on g protein-mediated signaling pathways. | Posner, BA., et al. 1999. J Biol Chem. 274: 31087-93. PMID: 10521509
- Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors. | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
- RGS9: a regulator of G-protein signalling with specific expression in rat and mouse striatum. | Thomas, EA., et al. 1998. J Neurosci Res. 52: 118-24. PMID: 9556034
- A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits. | Snow, BE., et al. 1998. Proc Natl Acad Sci U S A. 95: 13307-12. PMID: 9789084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RGS6/7 Antibody (F-10) | sc-271643 | 200 µg/ml | $322.00 | |||
RGS6/7 Antibody (F-10): m-IgG Fc BP-HRP Bundle | sc-529230 | 200 µg Ab; 10 µg BP | $361.00 | |||
RGS6/7 Antibody (F-10): m-IgGκ BP-HRP Bundle | sc-522007 | 200 µg Ab, 40 µg BP | $361.00 | |||
RGS6/7 Antibody (F-10): m-IgG1 BP-HRP Bundle | sc-543401 | 200 µg Ab; 20 µg BP | $361.00 | |||
RGS6/7 Antibody (F-10) AC | sc-271643 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RGS6/7 Antibody (F-10) HRP | sc-271643 HRP | 200 µg/ml | $322.00 | |||
RGS6/7 Antibody (F-10) FITC | sc-271643 FITC | 200 µg/ml | $336.00 | |||
RGS6/7 Antibody (F-10) PE | sc-271643 PE | 200 µg/ml | $349.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 488 | sc-271643 AF488 | 200 µg/ml | $364.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 546 | sc-271643 AF546 | 200 µg/ml | $364.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 594 | sc-271643 AF594 | 200 µg/ml | $364.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 647 | sc-271643 AF647 | 200 µg/ml | $364.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 680 | sc-271643 AF680 | 200 µg/ml | $364.00 | |||
RGS6/7 Antibody (F-10) Alexa Fluor® 790 | sc-271643 AF790 | 200 µg/ml | $364.00 |