RGS5 Antibody (B-4): sc-514184

RGS5 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RGS5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RGS5 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGS5 plays a crucial role in regulating G-protein signaling pathways, which are essential for various physiological processes, including cell growth, differentiation, and response to external stimuli. RGS5 localizes in vascular endothelial cells, where RGS5 modulates capillary morphogenesis and angiogenesis. This localization highlights RGS5′s potential role in vascular development and implications in diseases such as stroke, where abnormal angiogenesis can contribute to pathophysiological conditions. By shortening activated G protein lifespan, RGS5 helps maintain signaling pathway balance, making RGS5 a vital component in vascular function regulation and a promising therapeutic target.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RGS5 Antibody (B-4) References:

1. Emerging roles for RGS proteins in cell signalling.  |  Hepler, JR. 1999. Trends Pharmacol Sci. 20: 376-82. PMID: 10462761
2. Pericyte-specific expression of Rgs5: implications for PDGF and EDG receptor signaling during vascular maturation.  |  Cho, H., et al. 2003. FASEB J. 17: 440-2. PMID: 12514120
3. Regulators of G-protein signalling: multifunctional proteins with impact on signalling in the cardiovascular system.  |  Wieland, T. and Mittmann, C. 2003. Pharmacol Ther. 97: 95-115. PMID: 12559385
4. Transcription profiling of platelet-derived growth factor-B-deficient mouse embryos identifies RGS5 as a novel marker for pericytes and vascular smooth muscle cells.  |  Bondjers, C., et al. 2003. Am J Pathol. 162: 721-9. PMID: 12598306
5. Expression of regulator of G protein signalling protein 5 (RGS5) in the tumour vasculature of human renal cell carcinoma.  |  Furuya, M., et al. 2004. J Pathol. 203: 551-8. PMID: 15095478
6. Regulator of G protein signaling 5 marks peripheral arterial smooth muscle cells and is downregulated in atherosclerotic plaque.  |  Li, J., et al. 2004. J Vasc Surg. 40: 519-28. PMID: 15337883
7. Identification of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues.  |  Liang, Y., et al. 2005. FEBS J. 272: 791-9. PMID: 15670159
8. Beta adrenergic receptor-mediated atrial specific up-regulation of RGS5.  |  Jean-Baptiste, G., et al. 2005. Life Sci. 76: 1533-45. PMID: 15680317
9. Regulation of RGS5 GAP activity by GPSM3.  |  Zhao, P. and Chidiac, P. 2015. Mol Cell Biochem. 405: 33-40. PMID: 25842189
10. Hypertension-evoked RhoA activity in vascular smooth muscle cells requires RGS5.  |  Arnold, C., et al. 2018. FASEB J. 32: 2021-2035. PMID: 29208700
11. Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner.  |  Wang, X., et al. 2023. Int J Mol Sci. 24: PMID: 36982915
12. Cardiac myocytes express mRNA for ten RGS proteins: changes in RGS mRNA expression in ventricular myocytes and cultured atria.  |  Kardestuncer, T., et al. 1998. FEBS Lett. 438: 285-8. PMID: 9827562

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RGS5 Antibody (B-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RGS5 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RGS5 Antibody (B-4) Specifications

Alternate Product Name: RGS5

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 28-47

Epitope Target: RGS5

Epitope Species: human

Product Reactivity/Detection: RGS5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RGS5 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin