Anti-Rfp2 Antibody (E-6) is a mouse monoclonal IgG2a κ Rfp2 antibody, cited in 1 publications, provided at 200 µg/ml
raised against amino acids 212-309 mapping at the C-terminus of Rfp2 of human origin
Rfp2 Antibody (E-6) is recommended for detection of Rfp2 of human origin by WB, IP, IF and ELISA
Anti-Rfp2 Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rfp2 (E-6): sc-393257.
m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rfp2 Antibody (E-6) for WB applications. These reagents are now offered in bundles with Rfp2 Antibody (E-6) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Rfp2 Antibody (E-6) is a high quality monoclonal Rfp2 antibody (also designated Rfp2 antibody) suitable for the detection of the Rfp2 protein of human origin. Rfp2 Antibody (E-6) is available as both the non-conjugated anti-Rfp2 antibody form, as well as multiple conjugated forms of anti-Rfp2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B box-type zinc finger, one RING finger and three zinc-binding domains. Rfp2 (Ret finger protein 2), also known as TRIM13 (tripartite motif-containing 13), CAR, RNF77 or LEU5, is a 407 amino acid protein that belongs to the TRIM protein family and contains one B box-type zinc finger and one RING-type zinc finger. Existing as two alternatively spliced isoforms designated alpha and beta, Rfp2 is thought to act as a tumor suppressor that, when defective, may be involved in the development and progression of B-cell chronic lymphocytic leukemia. Additionally, Rfp2 may function as an E3 ubiquitin ligase that is involved in protein degradation pathways related to the ER-associated degradation (ERAD) pathway.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can I use this antibody to detect mRUBY2 tagged proteins for western blot?
Asked by: suzy
Thank you for your question. Rfp2 Antibody (E-6) is only recommended for detection of Rfp2 of human origin by WB, IP, IF and ELISA. Tagged Rfp2 fusion proteins may not be recognized by this antibody.
Answered by: Tech Support Europe
Date published: 2020-12-28
What blocking solution should I use with Rfp2 (E-6): sc-393257 monoclonal antibody for Western blot?
Asked by: DefinitelyNotMatt
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here:
Rated 5 out of
Satisfactory Western Blot data of Rfp2 expressionSatisfactory Western Blot data of Rfp2 expression in NTERA-2 cl.D1 and Y79 whole cell lysates and rat testis and human testis tissue -SCBT QC
Date published: 2015-02-04
Rated 5 out of
Nice Western Blot data of Rfp2 expression in IMRNice Western Blot data of Rfp2 expression in IMR-32 whole cell lysate. -SCBT QC
All trademarks are the property of their respective owners.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn