Rfp2 Antibody (E-6): sc-393257

Rfp2 Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Rfp2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rfp2 antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rfp2, also known as TRIM13, is a member of the tripartite motif (TRIM) family, characterized by a conserved TRIM domain that includes a coiled-coil region, a B box-type zinc finger, and a RING finger, which are crucial for its function as an E3 ubiquitin ligase. Rfp2 plays a significant role in the regulation of protein degradation pathways, particularly in the endoplasmic reticulum-associated degradation (ERAD) pathway, which is essential for maintaining cellular homeostasis by eliminating misfolded proteins. Rfp2 is also implicated in tumor suppression, and mutations in Rfp2 have been associated with the development and progression of B-cell chronic lymphocytic leukemia. Two alternatively spliced isoforms, alpha and beta, further highlight the complexity of Rfp2′s regulatory functions within the cell.

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Rfp2 Antibody (E-6) References:

1. The tripartite motif family identifies cell compartments.  |  Reymond, A., et al. 2001. EMBO J. 20: 2140-51. PMID: 11331580
2. RFP2, c13ORF1, and FAM10A4 are the most likely tumor suppressor gene candidates for B-cell chronic lymphocytic leukemia.  |  van Everdink, WJ., et al. 2003. Cancer Genet Cytogenet. 146: 48-57. PMID: 14499696
3. DLEU2 encodes an antisense RNA for the putative bicistronic RFP2/LEU5 gene in humans and mouse.  |  Corcoran, MM., et al. 2004. Genes Chromosomes Cancer. 40: 285-97. PMID: 15188451
4. Human RFP2 gene promoter: unique structure and unusual strength.  |  Skoblov, M., et al. 2006. Biochem Biophys Res Commun. 342: 859-66. PMID: 16499869
5. The RBCC gene RFP2 (Leu5) encodes a novel transmembrane E3 ubiquitin ligase involved in ERAD.  |  Lerner, M., et al. 2007. Mol Biol Cell. 18: 1670-82. PMID: 17314412
6. 13q deletions in lymphoid malignancies.  |  Liu, Y., et al. 1995. Blood. 86: 1911-5. PMID: 7655020
7. Chronic lymphocytic leukemia cells with allelic deletions at 13q14 commonly have one intact RB1 gene: evidence for a role of an adjacent locus.  |  Liu, Y., et al. 1993. Proc Natl Acad Sci U S A. 90: 8697-701. PMID: 8378351
8. A cosmid and cDNA fine physical map of a human chromosome 13q14 region frequently lost in B-cell chronic lymphocytic leukemia and identification of a new putative tumor suppressor gene, Leu5.  |  Kapanadze, B., et al. 1998. FEBS Lett. 426: 266-70. PMID: 9599022

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rfp2 Antibody (E-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Rfp2 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rfp2 Antibody (E-6) Specifications

Alternate Product Name: Rfp2

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 212-309

Epitope Target: Rfp2

Epitope Species: human

Product Reactivity/Detection: Rfp2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Rfp2 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin