Rex-1 Antibody (F-1): sc-514643

Rex-1 Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rex-1 of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Rex-1 (F-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rex-1, also known as zinc finger protein 42 (ZFP42), is an acidic zinc finger protein characterized by four repeats of the zinc finger nucleic acid-binding motif and a potential acidic activator domain, indicating a role as a regulatory protein. Rex-1 is predominantly localized in the nucleus, where Rex-1 plays a crucial role in maintaining embryonic stem cell pluripotency and shows high expression in undifferentiated murine F9 teratocarcinoma cells. Rex-1 expression undergoes downregulation during differentiation processes, such as retinoic acid introduction, highlighting Rex-1′s importance in stem cell biology. Additionally, various transcription factors modulate Rex-1 expression; Oct-3/4 can either activate or repress Rex-1 promoter depending on cellular context, while Oct-6 decreases Rex-1 expression. Such intricate regulation underscores Rex-1′s significance in developmental processes and potential implications in stem cell research and regenerative medicine.

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Rex-1 Antibody (F-1) References:

1. Retinoic acid induces parietal endoderm but not primitive endoderm and visceral endoderm differentiation in F9 teratocarcinoma stem cells with a targeted deletion of the Rex-1 (Zfp-42) gene.  |  Thompson, JR. and Gudas, LJ. 2002. Mol Cell Endocrinol. 195: 119-33. PMID: 12354678
2. Effect of nicotine on Oct-4 and Rex-1 expression of mouse embryonic stem cells.  |  Zhang, H., et al. 2005. Reprod Toxicol. 19: 473-8. PMID: 15749260
3. Regulation of the pluripotency marker Rex-1 by Nanog and Sox2.  |  Shi, W., et al. 2006. J Biol Chem. 281: 23319-25. PMID: 16714766
4. Specific expression of a retinoic acid-regulated, zinc-finger gene, Rex-1, in preimplantation embryos, trophoblast and spermatocytes.  |  Rogers, MB., et al. 1991. Development. 113: 815-24. PMID: 1821852
5. Association of Rex-1 to target genes supports its interaction with Polycomb function.  |  Garcia-Tuñon, I., et al. 2011. Stem Cell Res. 7: 1-16. PMID: 21530438
6. Knockdown of the potential cancer stem-like cell marker Rex-1 improves chemotherapeutic effects in gliomas.  |  Kim, BS., et al. 2011. Hum Gene Ther. 22: 1551-62. PMID: 21810014
7. Expression of REX-1, a gene containing zinc finger motifs, is rapidly reduced by retinoic acid in F9 teratocarcinoma cells.  |  Hosler, BA., et al. 1989. Mol Cell Biol. 9: 5623-9. PMID: 2511439
8. The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells.  |  Rosfjord, E. and Rizzino, A. 1994. Biochem Biophys Res Commun. 203: 1795-802. PMID: 7945330
9. An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells.  |  Hosler, BA., et al. 1993. Mol Cell Biol. 13: 2919-28. PMID: 8474450
10. Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site.  |  Ben-Shushan, E., et al. 1998. Mol Cell Biol. 18: 1866-78. PMID: 9528758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rex-1 Antibody (F-1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Rex-1 Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rex-1 Antibody (F-1) Specifications

Alternate Product Name: Rex-1

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 34-58

Epitope Target: Rex-1

Epitope Species: mouse

Product Reactivity/Detection: Rex-1

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Rex-1 Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Rex-1 antibody is Rex-1 Antibody (E-11): sc-377095.