Rev-erbα Antibody (RS-14): sc-100910

Rev-erbα Antibody (RS-14) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rev-erbα in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Rev-erbα antibody (RS-14) is offered in a non-conjugated form, allowing for versatile experimental use. Rev-erbα, an orphan nuclear receptor, plays a crucial role in regulating circadian rhythms and metabolic processes by acting as a transcriptional repressor. Rev-erbα (RS-14) antibody recognizes the protein primarily in the nucleus, where Rev-erbα binds to specific DNA sequences to modulate gene expression. Rev-erbα′s nuclear localization enables direct influence on the transcription of genes involved in the circadian clock and energy homeostasis, thereby impacting physiological processes such as sleep-wake cycles and metabolism. Rev-erbα interacts with various co-regulators, including the nuclear receptor co-activator 5 (NCOA5), which enhances transcriptional activity, further underscoring significance in cellular regulation. This multifaceted role makes Rev-erbα a vital target for research in chronobiology and metabolic disorders.

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Rev-erbα Antibody (RS-14) References:

1. CIA, a novel estrogen receptor coactivator with a bifunctional nuclear receptor interacting determinant.  |  Sauvé, F., et al. 2001. Mol Cell Biol. 21: 343-53. PMID: 11113208
2. Rev-erbalpha upregulates NF-kappaB-responsive genes in vascular smooth muscle cells.  |  Migita, H., et al. 2004. FEBS Lett. 561: 69-74. PMID: 15013753
3. Aberrant expression of myosin isoforms in skeletal muscles from mice lacking the rev-erbAalpha orphan receptor gene.  |  Pircher, P., et al. 2005. Am J Physiol Regul Integr Comp Physiol. 288: R482-90. PMID: 15374821
4. The role of the orphan nuclear receptor Rev-Erb alpha in adipocyte differentiation and function.  |  Laitinen, S., et al. 2005. Biochimie. 87: 21-5. PMID: 15733732
5. Genomic organization of the human thyroid hormone receptor alpha (c-erbA-1) gene.  |  Laudet, V., et al. 1991. Nucleic Acids Res. 19: 1105-12. PMID: 1850510
6. REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.  |  Wang, S., et al. 2018. Nat Commun. 9: 4246. PMID: 30315268
7. The circadian clock protein Rev-erbα provides neuroprotection and attenuates neuroinflammation against Parkinson's disease via the microglial NLRP3 inflammasome.  |  Kou, L., et al. 2022. J Neuroinflammation. 19: 133. PMID: 35668454
8. Microglial REV-ERBα regulates inflammation and lipid droplet formation to drive tauopathy in male mice.  |  Lee, J., et al. 2023. Nat Commun. 14: 5197. PMID: 37626048
9. Inhibiting Rev-erbα-mediated ferroptosis alleviates susceptibility to myocardial ischemia-reperfusion injury in type 2 diabetes.  |  Huang, Q., et al. 2023. Free Radic Biol Med. 209: 135-150. PMID: 37805047
10. Functional inversion of circadian regulator REV-ERBα leads to tumorigenic gene reprogramming.  |  Yang, Y., et al. 2024. Proc Natl Acad Sci U S A. 121: e2411321121. PMID: 39383000
11. A new orphan member of the nuclear hormone receptor superfamily closely related to Rev-Erb.  |  Dumas, B., et al. 1994. Mol Endocrinol. 8: 996-1005. PMID: 7997240
12. Structural elements of an orphan nuclear receptor-DNA complex.  |  Zhao, Q., et al. 1998. Mol Cell. 1: 849-61. PMID: 9660968

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rev-erbα Antibody (RS-14) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Rev-erbα Antibody (RS-14) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rev-erbα Antibody (RS-14) Specifications

Alternate Product Name: Rev-erbα

Product Clone Name: RS-14

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-615

Epitope Target: Rev-erbα

Epitope Species: human

Product Reactivity/Detection: Rev-erbα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Rev-erbα Antibody (RS-14): N/A

The preferred Rev-erbα antibody is Rev-erbα Antibody (E-12): sc-393215.