Rev-erbα Antibody (E-12): sc-393215
- Rev-erbα Antibody (E-12) is a mouse monoclonal IgG1 κ Rev-erbα antibody, cited in 10 publications, provided at 200 µg/ml
- raised against amino acids 21-69 mapping near the N-terminus of Rev-erbα of human origin
- Rev-erb alpha Antibody (E-12) is recommended for detection of Rev-erbα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Rev-erb alpha Antibody (E-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-393215 X, 200 µg/0.1 ml)
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rev-erbα Antibody (E-12) for WB applications. These reagents are now offered in bundles with Rev-erbα Antibody (E-12) (see ordering information below).
Rev-erbα Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rev-erbα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Rev-erbα (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rev-erbα, also known as Nuclear Receptor Subfamily 1 Group D Member 1, plays a crucial role in regulating circadian rhythms and metabolic processes by acting as a transcriptional repressor. Rev-erbα is primarily located in the nucleus, where Rev-erbα binds to specific DNA sequences to modulate gene expression. Nuclear localization enables Rev-erbα to interact with other nuclear proteins and transcription factors, thereby influencing the expression of genes involved in circadian rhythm regulation and lipid metabolism. Rev-erbα interacts with co-activator NCOA5, which enhances transcriptional activity, highlighting Rev-erbα′s importance in the intricate network of gene regulation. Both Rev-erbα and Rev-erbβ are integral to maintaining homeostasis within the body, making these proteins significant targets for research in metabolic disorders and circadian rhythm-related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rev-erbα Antibody (E-12) References:
- Rev-erbα and the circadian transcriptional regulation of metabolism. | Gerhart-Hines, Z. and Lazar, MA. 2015. Diabetes Obes Metab. 17 Suppl 1: 12-6. PMID: 26332963
- Role of the clock gene Rev-erbα in metabolism and in the endocrine pancreas. | Vieira, E., et al. 2015. Diabetes Obes Metab. 17 Suppl 1: 106-14. PMID: 26332975
- Pharmacological activation of REV-ERBα improves nonalcoholic steatohepatitis by regulating intestinal permeability. | Ni, Y., et al. 2021. Metabolism. 114: 154409. PMID: 33096076
- Dysregulation of REV-ERBα impairs GABAergic function and promotes epileptic seizures in preclinical models. | Zhang, T., et al. 2021. Nat Commun. 12: 1216. PMID: 33619249
- REV-ERBα regulates age-related and oxidative stress-induced degeneration in retinal pigment epithelium via NRF2. | Huang, S., et al. 2022. Redox Biol. 51: 102261. PMID: 35176707
- Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization. | Wang, Q., et al. 2023. Nat Commun. 14: 1295. PMID: 36894533
- Cardiomyocyte-specific disruption of the circadian BMAL1-REV-ERBα/β regulatory network impacts distinct miRNA species in the murine heart. | Latimer, MN., et al. 2023. Commun Biol. 6: 1149. PMID: 37952007
- Functional Characterization of Circadian Nuclear Receptors REV-ERBα and REV-ERBβ in Human Osteosarcoma Cell Cultures. | Cho, H., et al. 2024. Int J Mol Sci. 25: PMID: 38255844
- Deficiency of circadian clock gene Bmal1 exacerbates noncanonical inflammasome-mediated pyroptosis and lethality via Rev-erbα-C/EBPβ-SAA1 axis. | Shim, DW., et al. 2024. Exp Mol Med. 56: 370-382. PMID: 38297162
- Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling. | Zhong, D., et al. 2024. Cell Rep. 43: 114075. PMID: 38583151
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rev-erbα Antibody (E-12) | sc-393215 | 200 µg/ml | $322.00 | |||
Rev-erbα Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-523857 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rev-erbα Antibody (E-12): m-IgG1 BP-HRP Bundle | sc-533615 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rev-erbα Antibody (E-12) X | sc-393215 X | 200 µg/0.1 ml | $322.00 | |||
Rev-erbα Antibody (E-12) AC | sc-393215 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rev-erbα Antibody (E-12) HRP | sc-393215 HRP | 200 µg/ml | $322.00 | |||
Rev-erbα Antibody (E-12) FITC | sc-393215 FITC | 200 µg/ml | $336.00 | |||
Rev-erbα Antibody (E-12) PE | sc-393215 PE | 200 µg/ml | $349.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 488 | sc-393215 AF488 | 200 µg/ml | $364.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 546 | sc-393215 AF546 | 200 µg/ml | $364.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 594 | sc-393215 AF594 | 200 µg/ml | $364.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 647 | sc-393215 AF647 | 200 µg/ml | $364.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 680 | sc-393215 AF680 | 200 µg/ml | $364.00 | |||
Rev-erbα Antibody (E-12) Alexa Fluor® 790 | sc-393215 AF790 | 200 µg/ml | $364.00 |