Retinal RX Antibody (H-7): sc-376837

Retinal RX Antibody (H-7) is a mouse monoclonal IgG2b antibody that detects Retinal RX in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Retinal RX, also known as RAX (retina and anterior neural fold homeobox), MCOP3, or RX, is a 346 amino acid protein that plays a pivotal role in eye development by regulating retinal cell specification and proliferation. Retinal RX (H-7) antibody recognizes a nuclear protein containing one OAR domain and one homeobox DNA-binding domain, essential for transcriptional regulation and gene expression control. The homeobox DNA-binding domain, a conserved 60 amino acid motif, enables Retinal RX to bind DNA through a helix-turn-helix structure, influencing genes critical for eye formation. Defects in the gene encoding Retinal RX can lead to microphthalmia isolated type 3 (MCOP3), a disorder characterized by corneal and lens opacities as well as scarring of the retina and choroid, underscoring the significance of this protein in ocular health. Anti-Retinal RX antibody (H-7) serves as an invaluable tool for researchers studying retinal development and associated disorders.

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Retinal RX Antibody (H-7) References:

1. Mutations in the human RAX homeobox gene in a patient with anophthalmia and sclerocornea.  |  Voronina, VA., et al. 2004. Hum Mol Genet. 13: 315-22. PMID: 14662654
2. Interaction between RAX and PKR modulates the effect of ethanol on protein synthesis and survival of neurons.  |  Chen, G., et al. 2006. J Biol Chem. 281: 15909-15. PMID: 16574643
3. Rax regulates hypothalamic tanycyte differentiation and barrier function in mice.  |  Miranda-Angulo, AL., et al. 2014. J Comp Neurol. 522: 876-99. PMID: 23939786
4. Rax Homeoprotein Regulates Photoreceptor Cell Maturation and Survival in Association with Crx in the Postnatal Mouse Retina.  |  Irie, S., et al. 2015. Mol Cell Biol. 35: 2583-96. PMID: 25986607
5. Autoregulation of retinal homeobox (rax) gene promoter activity through a highly conserved genomic element.  |  Kelly, LE., et al. 2016. Genesis. 54: 562-567. PMID: 27696680
6. A modulatory role of the Rax homeobox gene in mature pineal gland function: Investigating the photoneuroendocrine circadian system of a Rax conditional knockout mouse.  |  Rohde, K., et al. 2017. J Neurochem. 143: 100-111. PMID: 28675567
7. Identification of retinal homeobox (rax) gene-dependent genes by a microarray approach: The DNA endoglycosylase neil3 is a major downstream component of the rax genetic pathway.  |  Pan, Y., et al. 2018. Dev Dyn. 247: 1199-1210. PMID: 30311321
8. The C-terminus of the retinal homeobox (rax) gene product modulates transcription in a context-dependent manner.  |  Buescher, JL. and El-Hodiri, HM. 2019. Mol Vis. 25: 165-173. PMID: 30820152
9. Origin and evolution of the Rax homeobox gene by comprehensive evolutionary analysis.  |  Kon, T. and Furukawa, T. 2020. FEBS Open Bio. 10: 657-673. PMID: 32144893
10. The Rax homeoprotein in Müller glial cells is required for homeostasis maintenance of the postnatal mouse retina.  |  Yoshimoto, T., et al. 2023. J Biol Chem. 299: 105461. PMID: 37977220

The preferred Retinal RX antibody is Retinal RX Antibody (G-12): sc-271889.