Retinal RX Antibody (G-12): sc-271889
- Retinal RX Antibody (G-12) is a mouse monoclonal IgG1 κ, cited in 11 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 4-29 at the N-terminus of Retinal RX of human origin
- Anti-Retinal RX Antibody (G-12) is recommended for detection of Retinal RX of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including porcine
- TransCruz reagent for ChIP application (sc-271889 X, 200 µg/0.1 ml)
- Anti-Retinal RX Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Retinal RX Antibody (G-12). This work is in progress.
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Retinal RX Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Retinal RX in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Retinal RX antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinal RX, also known as RAX (retina and anterior neural fold homeobox), MCOP3, or RX, is a 346 amino acid protein that plays a pivotal role in eye development by regulating the specification and proliferation of retinal cells. This nuclear protein contains a homeobox DNA-binding domain, a 60 amino acid motif crucial for binding DNA through a helix-turn-helix structure, thereby influencing transcriptional regulation and gene expression. Retinal RX expression in developing eye tissue and adult retina is significant, as defects in the Retinal RX gene can lead to microphthalmia isolated type 3 (MCOP3), a disorder characterized by corneal and lens opacities as well as scarring of the retina and choroid. Retinal RX (G-12) monoclonal antibody serves as a valuable tool for researchers studying eye development and associated disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Retinal RX Antibody (G-12) References:
- Both PCE-1/RX and OTX/CRX interactions are necessary for photoreceptor-specific gene expression. | Kimura, A., et al. 2000. J Biol Chem. 275: 1152-60. PMID: 10625658
- Regulation of eye formation by the Rx and pax6 homeobox genes. | Mathers, PH. and Jamrich, M. 2000. Cell Mol Life Sci. 57: 186-94. PMID: 10766016
- Superactivation of Pax6-mediated transactivation from paired domain-binding sites by dna-independent recruitment of different homeodomain proteins. | Mikkola, I., et al. 2001. J Biol Chem. 276: 4109-18. PMID: 11069920
- Mutations in the human RAX homeobox gene in a patient with anophthalmia and sclerocornea. | Voronina, VA., et al. 2004. Hum Mol Genet. 13: 315-22. PMID: 14662654
- Molecular links among the causative genes for ocular malformation: Otx2 and Sox2 coregulate Rax expression. | Danno, H., et al. 2008. Proc Natl Acad Sci U S A. 105: 5408-13. PMID: 18385377
- Confirmation of RAX gene involvement in human anophthalmia. | Lequeux, L., et al. 2008. Clin Genet. 74: 392-5. PMID: 18783408
- Sequence alterations in RX in patients with microphthalmia, anophthalmia, and coloboma. | London, NJ., et al. 2009. Mol Vis. 15: 162-7. PMID: 19158959
- An essential role for Rax in retina and neuroendocrine system development. | Muranishi, Y., et al. 2012. Dev Growth Differ. 54: 341-8. PMID: 22524605
- Conserved roles of Rax/rx3 genes in hypothalamus and pituitary development. | De Souza, FSJ. and Placzek, M. 2021. Int J Dev Biol. 65: 195-205. PMID: 32930382
- Resveratrol inhibits neural apoptosis and regulates RAX/P-PKR expression in retina of diabetic rats. | Zeng, K., et al. 2022. Nutr Neurosci. 25: 2560-2569. PMID: 34693895
- rax, a novel paired-type homeobox gene, shows expression in the anterior neural fold and developing retina. | Furukawa, T., et al. 1997. Proc Natl Acad Sci U S A. 94: 3088-93. PMID: 9096350
- The Rx homeobox gene is essential for vertebrate eye development. | Mathers, PH., et al. 1997. Nature. 387: 603-7. PMID: 9177348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Retinal RX Antibody (G-12) | sc-271889 | 200 µg/ml | $316.00 | |||
Retinal RX Antibody (G-12) X | sc-271889 X | 200 µg/0.1 ml | $316.00 | |||
Retinal RX Antibody (G-12) AC | sc-271889 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Retinal RX Antibody (G-12) HRP | sc-271889 HRP | 200 µg/ml | $316.00 | |||
Retinal RX Antibody (G-12) FITC | sc-271889 FITC | 200 µg/ml | $330.00 | |||
Retinal RX Antibody (G-12) PE | sc-271889 PE | 200 µg/ml | $343.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 488 | sc-271889 AF488 | 200 µg/ml | $357.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 546 | sc-271889 AF546 | 200 µg/ml | $357.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 594 | sc-271889 AF594 | 200 µg/ml | $357.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 647 | sc-271889 AF647 | 200 µg/ml | $357.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 680 | sc-271889 AF680 | 200 µg/ml | $357.00 | |||
Retinal RX Antibody (G-12) Alexa Fluor® 790 | sc-271889 AF790 | 200 µg/ml | $357.00 | |||
Retinal RX (G-12) Neutralizing Peptide | sc-271889 P | 100 µg/0.5 ml | $68.00 |