REP-2 Antibody (E-4): sc-398605
- REP-2 Antibody (E-4) is a mouse monoclonal IgG2a κ REP-2 antibody provided at 200 µg/ml
- raised against amino acids 58-97 mapping near the N-terminus of REP-2 of mouse origin
- REP-2 Antibody (E-4) is recommended for detection of REP-2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-REP-2 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for REP-2 Antibody (E-4) for WB applications. These reagents are now offered in bundles with REP-2 Antibody (E-4) (see ordering information below).
REP-2 Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects REP-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). REP-2 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. REP-2 protein plays a crucial role in the prenylation process of Rab proteins, which is essential for their proper localization and function within the cell. Specifically, newly synthesized Rab proteins are bound to Rab escort proteins (REP) and presented to Rab geranylgeranyltransferase (GGTase) type II, which facilitates the addition of geranylgeranyl groups to the carboxy-terminal cysteine residues of Rab proteins. This modification is vital as Rab proteins can associate with specific membranes, thereby regulating intracellular trafficking and signaling pathways. Notably, REP-2 can bind to several Rab proteins with an affinity comparable to REP-1, potentially serving as a compensatory mechanism to mitigate tissue abnormalities in conditions such as choroideraemia, where mutations in the REP-1 gene lead to severe retinal degeneration. REP-2′s ability to functionally substitute for REP-1 underscores its importance in maintaining cellular homeostasis and preventing disease onset.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
REP-2 Antibody (E-4) References:
- Visual impairment and REP-1 gene mutations in Japanese choroideremia patients. | Hayakawa, M., et al. 1999. Ophthalmic Genet. 20: 107-15. PMID: 10420196
- REP-1 gene mutations in Japanese patients with choroideremia. | Fujiki, K., et al. 1999. Graefes Arch Clin Exp Ophthalmol. 237: 735-40. PMID: 10447648
- Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase. | Alexandrov, K., et al. 1999. Eur J Biochem. 265: 160-70. PMID: 10491170
- [Clinical and genetic features of choroideremia]. | Ohba, N. and Isashiki, Y. 1999. Nippon Ganka Gakkai Zasshi. 103: 773-81. PMID: 10589235
- Crystal structure of Rab geranylgeranyltransferase at 2.0 A resolution. | Zhang, H., et al. 2000. Structure. 8: 241-51. PMID: 10745007
- Membrane targeting of a Rab GTPase that fails to associate with Rab escort protein (REP) or guanine nucleotide dissociation inhibitor (GDI). | Overmeyer, JH., et al. 2001. J Biol Chem. 276: 20379-86. PMID: 11389151
- REP-2, a Rab escort protein encoded by the choroideremia-like gene. | Cremers, FP., et al. 1994. J Biol Chem. 269: 2111-7. PMID: 8294464
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REP-2 Antibody (E-4) | sc-398605 | 200 µg/ml | $316.00 | |||
REP-2 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-530868 | 200 µg Ab; 10 µg BP | $354.00 | |||
REP-2 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-524417 | 200 µg Ab, 40 µg BP | $354.00 | |||
REP-2 Antibody (E-4): m-IgG2a BP-HRP Bundle | sc-547615 | 200 µg Ab; 10 µg BP | $354.00 | |||
REP-2 Antibody (E-4) AC | sc-398605 AC | 500 µg/ml, 25% agarose | $416.00 | |||
REP-2 Antibody (E-4) HRP | sc-398605 HRP | 200 µg/ml | $316.00 | |||
REP-2 Antibody (E-4) FITC | sc-398605 FITC | 200 µg/ml | $330.00 | |||
REP-2 Antibody (E-4) PE | sc-398605 PE | 200 µg/ml | $343.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 488 | sc-398605 AF488 | 200 µg/ml | $357.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 546 | sc-398605 AF546 | 200 µg/ml | $357.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 594 | sc-398605 AF594 | 200 µg/ml | $357.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 647 | sc-398605 AF647 | 200 µg/ml | $357.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 680 | sc-398605 AF680 | 200 µg/ml | $357.00 | |||
REP-2 Antibody (E-4) Alexa Fluor® 790 | sc-398605 AF790 | 200 µg/ml | $357.00 |