REP-1 Antibody (2F1): sc-23905
- REP-1 Antibody (2F1) is a mouse monoclonal IgG1 κ REP-1 antibody, cited in 12 publications, provided at 200 µg/ml
- raised against recombinant human REP-1
- REP-1 Antibody (2F1) is recommended for detection of REP-1 of human origin by WB, IP, IF and IHC(P)
- Anti-REP-1 Antibody (2F1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for REP-1 Antibody (2F1) for WB and IHC(P) applications. These reagents are now offered in bundles with REP-1 Antibody (2F1) (see ordering information below).
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REP-1 Antibody (2F1) is a mouse monoclonal IgG1 kappa light chain antibody that detects REP-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-REP-1 antibody (2F1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. REP-1 plays a crucial role in the prenylation of Rab proteins, which is essential for their proper localization and function within the cell. Specifically, newly synthesized Rab proteins are bound to Rab escort proteins (REP), which present them to Rab geranylgeranyltransferase (GGTase) type II for modification on two carboxy terminal cysteine residues. This prenylation allows Rab proteins to associate with specific membranes, facilitating their involvement in intracellular trafficking and signaling pathways. The REP-1 gene, located on chromosome Xq21, is associated with choroideraemia, a hereditary eye disease that leads to progressive vision loss due to mutations that disrupt REP-1 function. Understanding REP-1′s role in Rab protein prenylation highlights its importance in cellular processes and potential as a target for therapeutic interventions in diseases linked to dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
REP-1 Antibody (2F1) References:
- Visual impairment and REP-1 gene mutations in Japanese choroideremia patients. | Hayakawa, M., et al. 1999. Ophthalmic Genet. 20: 107-15. PMID: 10420196
- REP-1 gene mutations in Japanese patients with choroideremia. | Fujiki, K., et al. 1999. Graefes Arch Clin Exp Ophthalmol. 237: 735-40. PMID: 10447648
- Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase. | Alexandrov, K., et al. 1999. Eur J Biochem. 265: 160-70. PMID: 10491170
- [Clinical and genetic features of choroideremia]. | Ohba, N. and Isashiki, Y. 1999. Nippon Ganka Gakkai Zasshi. 103: 773-81. PMID: 10589235
- Crystal structure of Rab geranylgeranyltransferase at 2.0 A resolution. | Zhang, H., et al. 2000. Structure. 8: 241-51. PMID: 10745007
- Membrane targeting of a Rab GTPase that fails to associate with Rab escort protein (REP) or guanine nucleotide dissociation inhibitor (GDI). | Overmeyer, JH., et al. 2001. J Biol Chem. 276: 20379-86. PMID: 11389151
- REP-2, a Rab escort protein encoded by the choroideremia-like gene. | Cremers, FP., et al. 1994. J Biol Chem. 269: 2111-7. PMID: 8294464
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REP-1 Antibody (2F1) | sc-23905 | 200 µg/ml | $316.00 | |||
REP-1 Antibody (2F1): m-IgG Fc BP-HRP Bundle | sc-526630 | 200 µg Ab; 10 µg BP | $354.00 | |||
REP-1 Antibody (2F1): m-IgG1 BP-HRP Bundle | sc-532003 | 200 µg Ab; 20 µg BP | $354.00 | |||
REP-1 Antibody (2F1) AC | sc-23905 AC | 500 µg/ml, 25% agarose | $416.00 | |||
REP-1 Antibody (2F1) HRP | sc-23905 HRP | 200 µg/ml | $316.00 | |||
REP-1 Antibody (2F1) FITC | sc-23905 FITC | 200 µg/ml | $330.00 | |||
REP-1 Antibody (2F1) PE | sc-23905 PE | 200 µg/ml | $343.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 488 | sc-23905 AF488 | 200 µg/ml | $357.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 546 | sc-23905 AF546 | 200 µg/ml | $357.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 594 | sc-23905 AF594 | 200 µg/ml | $357.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 647 | sc-23905 AF647 | 200 µg/ml | $357.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 680 | sc-23905 AF680 | 200 µg/ml | $357.00 | |||
REP-1 Antibody (2F1) Alexa Fluor® 790 | sc-23905 AF790 | 200 µg/ml | $357.00 |