Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Rent3b CRISPR/Cas9 KO Plasmid (h): sc-406904
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rent3b CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rent3b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rent3b Antibody (D-1): sc-514564
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rent3b CRISPR/Cas9 KO Plasmid (h) | sc-406904 | 20 µg | $397.00 |
Overview
UPF3B encodes Rent3b, a core component of the nonsense-mediated mRNA decay (NMD) machinery that safeguards transcriptome integrity by eliminating mRNAs containing premature termination codons and regulating subsets of physiological transcripts. Rent3b associates with exon junction complex-linked surveillance and interacts with NMD factors such as UPF2 and UPF1 to couple translation termination to targeted RNA turnover. Through these activities, UPF3B influences RNA quality control, gene expression homeostasis, and cellular stress responses tied to aberrant mRNA processing. Disruption of UPF3B-dependent NMD has been linked to neurodevelopmental phenotypes and altered expression programs relevant to neurological and other RNA-metabolism–associated disorders.
Rent3b CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UPF3B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UPF3B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UPF3B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rent3b protein expression.
This CRISPR knockout system enables efficient generation of UPF3B-deficient cell models for investigation of Rent3b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UPF3B exon(s) critical for Rent3b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UPF3B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rent3b CRISPR/Cas9 KO Plasmid (h) and Rent3b CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UPF3B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rent3b HDR Plasmid (h) and Rent3b HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UPF3B homology arms to support homology-directed repair at defined UPF3B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.