Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
REEP1 CRISPR/Cas9 KO Plasmid (h): sc-407088
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- REEP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the REEP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: REEP1 Antibody (A-8): sc-393242
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REEP1 CRISPR/Cas9 KO Plasmid (h) | sc-407088 | 20 µg | $397.00 |
Overview
REEP1 (receptor expression-enhancing protein 1) is an endoplasmic reticulum (ER)-resident membrane protein that helps shape tubular ER architecture and supports membrane trafficking, including interactions with microtubule-associated processes in neurons. It contributes to ER dynamics, ER–mitochondria communication, and maintenance of long axons where membrane organization and organelle distribution are tightly coupled to cellular homeostasis. REEP1 dysfunction has been linked to inherited neurodegenerative phenotypes, most prominently autosomal dominant hereditary spastic paraplegia (SPG31), and is studied in the context of axonopathy and motor neuron vulnerability. Experimental models commonly interrogate REEP1-dependent changes in ER morphology, mitochondrial distribution, and stress signaling pathways such as the unfolded protein response.
REEP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the REEP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the REEP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the REEP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish REEP1 protein expression.
This CRISPR knockout system enables efficient generation of REEP1-deficient cell models for investigation of REEP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting REEP1 exon(s) critical for REEP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple REEP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by REEP1 CRISPR/Cas9 KO Plasmid (h) and REEP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the REEP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by REEP1 HDR Plasmid (h) and REEP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by REEP1 homology arms to support homology-directed repair at defined REEP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.