REDD-1 Antibody (B-3): sc-376671

REDD-1 Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects REDD-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). REDD-1 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. REDD-1, also known as DNA-damage-inducible transcript 4, dig2, or RTP801, plays a critical role in regulating reactive oxygen species (ROS), which are essential for maintaining cellular homeostasis and signaling. REDD-1 is primarily located in the cytoplasm and translocates to the nucleus under stress conditions, where REDD-1 can influence gene expression related to apoptosis and cellular stress responses. This localization enables REDD-1 to act as a mediator between cellular stress signals and downstream effects on cell survival and metabolism. As a hypoxia-inducible factor (HIF-1) responsive gene, REDD-1 shows strong upregulation in ischemic neuronal cells, underscoring REDD-1′s importance in the pathogenesis of ischemic diseases. REDD-1 negatively regulates the mammalian target of rapamycin (mTOR), a key serine/threonine kinase that integrates various extracellular and intracellular signals, making REDD-1 vital for cellular growth and metabolism. REDD-1 absence has been linked to retinopathy development, a leading cause of blindness, highlighting REDD-1′s potential as a therapeutic target in related diseases.

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REDD-1 Antibody (B-3) References:

1. Identification of a novel hypoxia-inducible factor 1-responsive gene, RTP801, involved in apoptosis.  |  Shoshani, T., et al. 2002. Mol Cell Biol. 22: 2283-93. PMID: 11884613
2. Identification of amyloid beta-peptide responsive genes by cDNA microarray technology: involvement of RTP801 in amyloid beta-peptide toxicity.  |  Kim, JR., et al. 2003. Exp Mol Med. 35: 403-11. PMID: 14646594
3. Sp1-dependent regulation of the RTP801 promoter and its application to hypoxia-inducible VEGF plasmid for ischemic disease.  |  Lee, M., et al. 2004. Pharm Res. 21: 736-41. PMID: 15180327
4. Regulation of mTOR function in response to hypoxia by REDD1 and the TSC1/TSC2 tumor suppressor complex.  |  Brugarolas, J., et al. 2004. Genes Dev. 18: 2893-904. PMID: 15545625
5. REDD1 integrates hypoxia-mediated survival signaling downstream of phosphatidylinositol 3-kinase.  |  Schwarzer, R., et al. 2005. Oncogene. 24: 1138-49. PMID: 15592522
6. The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway.  |  Corradetti, MN., et al. 2005. J Biol Chem. 280: 9769-72. PMID: 15632201
7. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization.  |  Zhao, H., et al. 2016. Int J Mol Sci. 17: PMID: 27898044
8. REDD1/autophagy pathway promotes thromboinflammation and fibrosis in human systemic lupus erythematosus (SLE) through NETs decorated with tissue factor (TF) and interleukin-17A (IL-17A).  |  Frangou, E., et al. 2019. Ann Rheum Dis. 78: 238-248. PMID: 30563869
9. The stress response protein REDD1 as a causal factor for oxidative stress in diabetic retinopathy.  |  Miller, WP., et al. 2021. Free Radic Biol Med. 165: 127-136. PMID: 33524531
10. REDD1 attenuates hepatic stellate cell activation and liver fibrosis via inhibiting of TGF-β/Smad signaling pathway.  |  Cho, SS., et al. 2021. Free Radic Biol Med. 176: 246-256. PMID: 34614448
11. Helicobacter pylori-induced REDD1 modulates Th17 cell responses that contribute to gastritis.  |  Yan, ZB., et al. 2021. Clin Sci (Lond). 135: 2541-2558. PMID: 34730176
12. Activation of Disulfide Redox Switch in REDD1 Promotes Oxidative Stress Under Hyperglycemic Conditions.  |  Miller, WP., et al. 2022. Diabetes. 71: 2764-2776. PMID: 36170669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-REDD-1 Antibody (B-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-REDD-1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

REDD-1 Antibody (B-3) Specifications

Alternate Product Name: REDD-1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 31-50

Epitope Target: REDD-1

Epitope Species: human

Product Reactivity/Detection: REDD-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for REDD-1 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred REDD-1 antibody is REDD-1 Antibody (A-4): sc-271158.