REDD-1 Antibody (A-4): sc-271158
- REDD-1 Antibody (A-4) is a mouse monoclonal IgG1 κ REDD-1 antibody provided at 200 µg/ml
- raised against amino acids 28-137 mapping within an internal region of REDD-1 of human origin
- REDD-1 Antibody (A-4) is recommended for detection of REDD-1 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-REDD-1 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for REDD-1 Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with REDD-1 Antibody (A-4) (see ordering information below).
QUICK LINKS
REDD-1 Antibody (A-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects REDD-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). REDD-1 (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. REDD-1, also known as DNA Damage Inducible Transcript 4, plays a crucial role in cellular response to stress, particularly in regulating reactive oxygen species (ROS). By modulating ROS levels, REDD-1 is essential for maintaining cellular homeostasis and preventing oxidative damage, which can lead to apoptosis and contribute to pathogenesis of ischemic diseases. Notably, REDD-1 is an HIF-1-responsive gene significantly upregulated in hypoxic conditions, particularly in neuronal cells, highlighting REDD-1′s importance in adaptive responses to low oxygen levels. This makes REDD-1 a valuable target for research into neuroprotection and ischemic injury, as well as a potential biomarker for various diseases associated with oxidative stress.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
REDD-1 Antibody (A-4) References:
- DNA damage-inducible transcript 4 (DDIT4) mediates methamphetamine-induced autophagy and apoptosis through mTOR signaling pathway in cardiomyocytes. | Chen, R., et al. 2016. Toxicol Appl Pharmacol. 295: 1-11. PMID: 26825372
- Emerging role for regulated in development and DNA damage 1 (REDD1) in the regulation of skeletal muscle metabolism. | Gordon, BS., et al. 2016. Am J Physiol Endocrinol Metab. 311: E157-74. PMID: 27189933
- DNA Damage Inducible Transcript 4 Gene: The Switch of the Metabolism as Potential Target in Cancer. | Tirado-Hurtado, I., et al. 2018. Front Oncol. 8: 106. PMID: 29707520
- Blocking the REDD1/TXNIP axis ameliorates LPS-induced vascular endothelial cell injury through repressing oxidative stress and apoptosis. | Hou, X., et al. 2019. Am J Physiol Cell Physiol. 316: C104-C110. PMID: 30485138
- A Key Role of DNA Damage-Inducible Transcript 4 (DDIT4) Connects Autophagy and GLUT3-Mediated Stemness To Desensitize Temozolomide Efficacy in Glioblastomas. | Ho, KH., et al. 2020. Neurotherapeutics. 17: 1212-1227. PMID: 31916238
- Redd1 knockdown prevents doxorubicin-induced cardiac senescence. | Huang, P., et al. 2021. Aging (Albany NY). 13: 13788-13806. PMID: 33962393
- REDD1 promotes obesity-induced metabolic dysfunction via atypical NF-κB activation. | Lee, DK., et al. 2022. Nat Commun. 13: 6303. PMID: 36272977
- The stress-responsive protein REDD1 and its pathophysiological functions. | Kim, JY., et al. 2023. Exp Mol Med. 55: 1933-1944. PMID: 37653030
- Nuclear overexpression of DNA damage-inducible transcript 4 (DDIT4) is associated with aggressive tumor behavior in patients with pancreatic tumors. | Tajik, F., et al. 2023. Sci Rep. 13: 19403. PMID: 37938616
- Lipoteichoic acid restrains macrophage senescence via β-catenin/FOXO1/REDD1 pathway in age-related osteoporosis. | Cheng, W., et al. 2024. Aging Cell. 23: e14072. PMID: 38126583
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REDD-1 Antibody (A-4) | sc-271158 | 200 µg/ml | $322.00 | |||
REDD-1 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-529131 | 200 µg Ab; 10 µg BP | $361.00 | |||
REDD-1 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-521863 | 200 µg Ab, 40 µg BP | $361.00 | |||
REDD-1 Antibody (A-4): m-IgG1 BP-HRP Bundle | sc-543339 | 200 µg Ab; 20 µg BP | $361.00 | |||
REDD-1 Antibody (A-4) AC | sc-271158 AC | 500 µg/ml, 25% agarose | $424.00 | |||
REDD-1 Antibody (A-4) HRP | sc-271158 HRP | 200 µg/ml | $322.00 | |||
REDD-1 Antibody (A-4) FITC | sc-271158 FITC | 200 µg/ml | $336.00 | |||
REDD-1 Antibody (A-4) PE | sc-271158 PE | 200 µg/ml | $349.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 488 | sc-271158 AF488 | 200 µg/ml | $364.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 546 | sc-271158 AF546 | 200 µg/ml | $364.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 594 | sc-271158 AF594 | 200 µg/ml | $364.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 647 | sc-271158 AF647 | 200 µg/ml | $364.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 680 | sc-271158 AF680 | 200 µg/ml | $364.00 | |||
REDD-1 Antibody (A-4) Alexa Fluor® 790 | sc-271158 AF790 | 200 µg/ml | $364.00 |