RecQL5 Antibody (C-9): sc-515050

RecQL5 Antibody (C-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects RecQL5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RecQL5 antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RecQL5 plays a crucial role in maintaining genomic stability, particularly during DNA replication and repair processes, which is vital for preventing mutations that can lead to cancer and other genetic disorders. As a member of the RecQ helicase family, which includes other important proteins such as WRN and BLM, RecQL5 is involved in unwinding DNA, thereby facilitating proper functioning of cellular mechanisms that safeguard genetic integrity. Differential expression of RecQ helicases, including RecQL5, is essential in cells that are actively proliferating or undergoing transformation, as RecQL5 helps manage increased demand for DNA repair and replication. The gene encoding RecQL5 is located on chromosome 17q25.1, and while specific associations with genetic disorders are not fully understood, functional importance in cellular processes underscores RecQL5′s relevance in research and clinical applications.

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RecQL5 Antibody (C-9) References:

1. Mutations in RECQL4 cause a subset of cases of Rothmund-Thomson syndrome.  |  Kitao, S., et al. 1999. Nat Genet. 22: 82-4. PMID: 10319867
2. Covalent modification of the Werner's syndrome gene product with the ubiquitin-related protein, SUMO-1.  |  Kawabe, Y., et al. 2000. J Biol Chem. 275: 20963-6. PMID: 10806190
3. Differential regulation of human RecQ family helicases in cell transformation and cell cycle.  |  Kawabe, T., et al. 2000. Oncogene. 19: 4764-72. PMID: 11032027
4. Cloning, genomic structure and chromosomal localization of the gene encoding mouse DNA helicase RecQ helicase protein-like 4.  |  Ohhata, T., et al. 2000. Gene. 261: 251-8. PMID: 11167012
5. RECQL5 helicase: connections to DNA recombination and RNA polymerase II transcription.  |  Aygün, O. and Svejstrup, JQ. 2010. DNA Repair (Amst). 9: 345-53. PMID: 20080450
6. Human RECQL5: guarding the crossroads of DNA replication and transcription and providing backup capability.  |  Popuri, V., et al. 2013. Crit Rev Biochem Mol Biol. 48: 289-99. PMID: 23627586
7. Recql5 protects against lipopolysaccharide/D-galactosamine-induced liver injury in mice.  |  Liao, WQ., et al. 2015. World J Gastroenterol. 21: 10375-84. PMID: 26420964
8. Decreased RECQL5 correlated with disease progression of osteosarcoma.  |  Wu, J., et al. 2015. Biochem Biophys Res Commun. 467: 617-22. PMID: 26499077
9. RECQL5 plays co-operative and complementary roles with WRN syndrome helicase.  |  Popuri, V., et al. 2017. Nucleic Acids Res. 45: 1566. PMID: 28180303
10. RECQL5 at the Intersection of Replication and Transcription.  |  Hamadeh, Z. and Lansdorp, P. 2020. Front Cell Dev Biol. 8: 324. PMID: 32523948
11. RECQL5 KIX domain splicing isoforms have distinct functions in transcription repression and DNA damage response.  |  Ding, D., et al. 2021. DNA Repair (Amst). 97: 103007. PMID: 33197722
12. Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12.  |  Seki, M., et al. 1994. Nucleic Acids Res. 22: 4566-73. PMID: 7527136

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RecQL5 Antibody (C-9) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-RecQL5 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RecQL5 Antibody (C-9) Specifications

Alternate Product Name: RecQL5

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 194-291

Epitope Target: RecQL5

Epitope Species: human

Product Reactivity/Detection: RecQL5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for RecQL5 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin