RecQL4 Antibody (B-3): sc-518189
- RecQL4 Antibody (B-3) is a mouse monoclonal IgM κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 28-50 of RecQL4 of human origin
- recommended for detection of RecQL4 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RecQL4 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for RecQL4 Antibody (B-3) for WB applications. This reagent is now offered in a bundle with RecQL4 Antibody (B-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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RecQL4 Antibody (B-3) is a mouse monoclonal IgM kappa antibody that detects RecQL4 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RecQL4 plays a crucial role in maintaining genomic stability, particularly during DNA replication and repair processes, which is vital for preventing genomic instability that can lead to various diseases, including cancer. RecQL4 is primarily located in the nucleus, where RecQL4 participates in the unwinding of DNA, a necessary step for replication and repair. Proper functioning of RecQL4 is essential, as mutations or dysregulation of this helicase can result in conditions such as Rothmund-Thomson syndrome, characterized by developmental defects and increased cancer susceptibility. Anti-RecQL4 antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. The gene encoding human RecQL4 is located on chromosome 8q24.3, and RecQL4 expression is differentially regulated in response to cellular stress, highlighting RecQL4′s importance in cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RecQL4 Antibody (B-3) References:
- Mutations in RECQL4 cause a subset of cases of Rothmund-Thomson syndrome. | Kitao, S., et al. 1999. Nat Genet. 22: 82-4. PMID: 10319867
- Covalent modification of the Werner's syndrome gene product with the ubiquitin-related protein, SUMO-1. | Kawabe, Y., et al. 2000. J Biol Chem. 275: 20963-6. PMID: 10806190
- Differential regulation of human RecQ family helicases in cell transformation and cell cycle. | Kawabe, T., et al. 2000. Oncogene. 19: 4764-72. PMID: 11032027
- Cloning, genomic structure and chromosomal localization of the gene encoding mouse DNA helicase RecQ helicase protein-like 4. | Ohhata, T., et al. 2000. Gene. 261: 251-8. PMID: 11167012
- RECQL4 in genomic instability and aging. | Croteau, DL., et al. 2012. Trends Genet. 28: 624-31. PMID: 22940096
- RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer. | Lyu, G., et al. 2021. Cancer Biol Med. 18: 120-138. PMID: 33628589
- Human RecQL4 as a Novel Molecular Target for Cancer Therapy. | Balajee, AS. 2021. Cytogenet Genome Res. 161: 305-327. PMID: 34474412
- Role and Regulation of the RECQL4 Family during Genomic Integrity Maintenance. | Luong, TT. and Bernstein, KA. 2021. Genes (Basel). 12: PMID: 34946868
- RECQL4 Inhibits Radiation-Induced Tumor Immune Awakening via Suppressing the cGAS-STING Pathway in Hepatocellular Carcinoma. | Hong, W., et al. 2024. Adv Sci (Weinh). 11: e2308009. PMID: 38381090
- RECQL4 is not critical for firing of human DNA replication origins. | Padayachy, L., et al. 2024. Sci Rep. 14: 7708. PMID: 38565932
- Unraveling the mechanisms of RECQL4-mediated cervical cancer progression through the PI3K/AKT pathway. | Huo, W., et al. 2024. Transl Oncol. 50: 102146. PMID: 39378549
- Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12. | Seki, M., et al. 1994. Nucleic Acids Res. 22: 4566-73. PMID: 7527136
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RecQL4 Antibody (B-3) | sc-518189 | 200 µg/ml | $322.00 | |||
RecQL4 Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-550905 | 200 µg Ab; 40 µg BP | $361.00 | |||
RecQL4 Antibody (B-3) AC | sc-518189 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RecQL4 Antibody (B-3) HRP | sc-518189 HRP | 200 µg/ml | $322.00 | |||
RecQL4 Antibody (B-3) FITC | sc-518189 FITC | 200 µg/ml | $336.00 | |||
RecQL4 Antibody (B-3) PE | sc-518189 PE | 200 µg/ml | $349.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 488 | sc-518189 AF488 | 200 µg/ml | $364.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 546 | sc-518189 AF546 | 200 µg/ml | $364.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 594 | sc-518189 AF594 | 200 µg/ml | $364.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 647 | sc-518189 AF647 | 200 µg/ml | $364.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 680 | sc-518189 AF680 | 200 µg/ml | $364.00 | |||
RecQL4 Antibody (B-3) Alexa Fluor® 790 | sc-518189 AF790 | 200 µg/ml | $364.00 |