Recoverin Antibody (6A55CD6): sc-53520

Recoverin Antibody (6A55CD6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Recoverin protein of mouse, rat, and human origin by western blotting (WB) and immunofluorescence (IF). Recoverin (6A55CD6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Recoverin plays a crucial role in the phototransduction cascade, which is essential for converting light into electrical signals in the retina. Recoverin is primarily located in the photoreceptor cells of the retina, where Recoverin binds to rhodopsin kinase in a calcium-dependent manner, thereby regulating the activity of this kinase and facilitating the recovery of sensitivity after exposure to bright light. Recoverin′s ability to modulate rhodopsin kinase is vital for the adaptation of photoreceptor cells to varying light conditions, ensuring optimal visual function. Additionally, Recoverin is recognized as an autoantigen in cancer-associated retinopathy, a rare neurological syndrome linked to small-cell lung cancer, highlighting Recoverin′s significance in both normal physiology and disease states. Anti-Recoverin antibody (6A55CD6)′s versatility in applications such as western blotting (WB) and immunofluorescence (IF) makes anti-Recoverin antibody (6A55CD6) an invaluable tool for researchers studying retinal function and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Recoverin Antibody (6A55CD6) References:

1. Calcium-dependent regulation of rhodopsin phosphorylation.  |  Kawamura, S. 1999. Novartis Found Symp. 224: 208-18; discussion 218-24. PMID: 10614053
2. How can Ca2+ selectively activate recoverin in the presence of Mg2+? Surface plasmon resonance and FT-IR spectroscopic studies.  |  Ozawa, T., et al. 2000. Biochemistry. 39: 14495-503. PMID: 11087403
3. Retinal degeneration under the effect of antibodies to recoverin.  |  Bazhin, AV., et al. 2001. Bull Exp Biol Med. 131: 350-2. PMID: 11550024
4. The cancer-associated retinopathy antigen is a recoverin-like protein.  |  Thirkill, CE., et al. 1992. Invest Ophthalmol Vis Sci. 33: 2768-72. PMID: 1388144
5. Differential recruitment of glucocorticoid receptor phospho-isoforms to glucocorticoid-induced genes.  |  Blind, RD. and Garabedian, MJ. 2008. J Steroid Biochem Mol Biol. 109: 150-7. PMID: 18304804
6. Autoantibody targets and their cancer relationship in the pathogenicity of paraneoplastic retinopathy.  |  Adamus, G. 2009. Autoimmun Rev. 8: 410-4. PMID: 19168157
7. Insulin-mediated regulation of tyrosine aminotransferase in rat hepatoma cells: inhibition of transcription and inhibition of enzyme degradation.  |  Moore, PS. and Koontz, JW. 1989. Arch Biochem Biophys. 275: 486-95. PMID: 2574563
8. Cancer-associated retinopathy after anti-programmed death 1 (PD-1) antibody for treating hepatocellular carcinoma--a case report of a Chinese patient.  |  Chen, Q., et al. 2022. Am J Ophthalmol Case Rep. 25: 101370. PMID: 35243142
9. Recoverin, a photoreceptor-specific calcium-binding protein, is expressed by the tumor of a patient with cancer-associated retinopathy.  |  Polans, AS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9176-80. PMID: 7568096
10. Antibody reactions with retina and cancer-associated antigens in 10 patients with cancer-associated retinopathy.  |  Thirkill, CE., et al. 1993. Arch Ophthalmol. 111: 931-7. PMID: 8392327
11. Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines.  |  Matsubara, S., et al. 1996. Br J Cancer. 74: 1419-22. PMID: 8912538
12. Autophosphorylation and ADP regulate the Ca2+-dependent interaction of recoverin with rhodopsin kinase.  |  Satpaev, DK., et al. 1998. Biochemistry. 37: 10256-62. PMID: 9665733

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Recoverin Antibody (6A55CD6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Recoverin Antibody (6A55CD6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Recoverin Antibody (6A55CD6) Specifications

Alternate Product Name: Recoverin

Product Clone Name: 6A55CD6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Recoverin

Epitope Species: human

Product Reactivity/Detection: Recoverin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and ovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Recoverin Antibody (6A55CD6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin