RCK Antibody (E-8): sc-166590

RCK Antibody (E-8) is a mouse monoclonal IgG1 antibody that detects RCK in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RCK, also known as DDX6 or P54, is a crucial member of the DEAD-box RNA helicase family, characterized by common protein motifs and widespread presence in various tissues. RCK plays a vital role in RNA metabolism, functioning as an unwindase that exhibits ATP-dependent RNA unwinding activity and facilitates RNA decay in the 5′-3′ direction. RCK is integral to normal cellular processes such as splicing, export, and translation initiation, ensuring proper regulation of gene expression. Notably, mutations in the gene encoding RCK can lead to overexpression, transforming RCK into an oncogene that enhances expression of genes associated with cell growth and proliferation. Through unwindase activity, RCK primarily functions to downregulate mRNA expression, maintaining normal transcriptional levels within cells, which is essential for cellular homeostasis and function. Anti-RCK antibody (E-8) is an invaluable tool for researchers investigating RCK′s intricate roles in both normal physiology and disease states.

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RCK Antibody (E-8) References:

1. Overexpression of a DEAD box/RNA helicase protein, rck/p54, in human hepatocytes from patients with hepatitis C virus-related chronic hepatitis and its implication in hepatocellular carcinogenesis.  |  Miyaji, K., et al. 2003. J Viral Hepat. 10: 241-8. PMID: 12823589
2. Electrostatic interaction of a K+ channel RCK domain with charged membrane surfaces.  |  Ptak, CP., et al. 2005. Biochemistry. 44: 62-71. PMID: 15628846
3. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.  |  Chu, CY. and Rana, TM. 2006. PLoS Biol. 4: e210. PMID: 16756390
4. Human DEAD-box/RNA unwindase rck/p54 contributes to maintenance of cell growth by affecting cell cycle in cultured cells.  |  Akao, Y., et al. 2006. Int J Oncol. 29: 41-8. PMID: 16773183
5. Knockdown of RCK/p54 expression by RNAi inhibits proliferation of human colorectal cancer cells in vitro and in vivo.  |  Lin, F., et al. 2008. Cancer Biol Ther. 7: 1669-76. PMID: 18769115
6. Multiple binding of repressed mRNAs by the P-body protein Rck/p54.  |  Ernoult-Lange, M., et al. 2012. RNA. 18: 1702-15. PMID: 22836354
7. Analysis of Ca(2+)-binding sites in the MthK RCK domain by X-ray crystallography.  |  Smith, FJ. and Rothberg, BS. 2013. Methods Mol Biol. 998: 277-87. PMID: 23529438
8. Structural basis of allosteric interactions among Ca2+-binding sites in a K+ channel RCK domain.  |  Smith, FJ., et al. 2013. Nat Commun. 4: 2621. PMID: 24126388
9. Characterization of the molecular properties of KtrC, a second RCK domain that regulates a Ktr channel in Bacillus subtilis.  |  Rocha, R., et al. 2019. J Struct Biol. 205: 34-43. PMID: 30753894
10. xbx-4, a homolog of the Joubert syndrome gene FAM149B1, acts via the CCRK and RCK kinase cascade to regulate cilia morphology.  |  Maurya, AK. and Sengupta, P. 2021. Curr Biol. 31: 5642-5649.e5. PMID: 34731674

The preferred RCK antibody is RCK Antibody (E-12): sc-376433.