Anti-Rbx1 Antibody (E-11) is a mouse monoclonal IgG1 (kappa light chain) Rbx1 antibody provided at 200 µg/ml
raised against amino acids 44-108 mapping at the C-terminus of Rbx1 of human origin
Anti-Rbx1 Antibody (E-11) is recommended for detection of Rbx1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Rbx1 Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rbx1 (E-11): sc-393640.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Rbx1 Antibody (E-11) is a high quality monoclonal Rbx1 antibody (also designated Rbx1 antibody) suitable for the detection of the Rbx1 protein of mouse, rat and human origin. Rbx1 Antibody (E-11) is available as both the non-conjugated anti-Rbx1 antibody form, as well as multiple conjugated forms of anti-Rbx1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Rbx1 (also designated ROC1 and Hrt1) and the closely related protein Rbx2 (also designated ROC2) are RING finger containing homologs of the yeast protein APC11, a member of the anaphase-promoting complex (APC). Rbx1 was shown to be a component of the von Hippel-Lindau (VHL) transcription elongation complex, which includes VHL, Elongin B, Elongin C and Cullin-2. Rbx1 interacts with Cullin-1 in the SCF (Skp1-Cdc53-F-box protein) ubiquitin ligase complex (2-4). Rbx1 functions as a common subunit of SCF complexes required for ubiquination of various proteins including yeast G1 cyclins, IkBa and b-catenin (2-4). Rbx1 was shown to enhance the ubiquitin ligase activity of the VHL/Cullin-2 complex, and of the SCF/Cullin-1 complex.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 5 out of
Good for WBThis antibody works great for Western blot using human cell whole cell lysate.
Date published: 2018-02-21
Rated 5 out of
Xiong Lab from
Very clear band and clean background for WBThis worked well at 1:1000-2000, very clear band and very clean background.
WB (HeLa cells)
1:1000 in 5% BSA in PBST
G anti-mouse 1:3000
Date published: 2017-07-05
Rated 5 out of
Great immunofluorescence nuclear and cytoplasmicGreat immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-21
Rated 5 out of
Satisfactory Western Blot data of Rbx1 expressionSatisfactory Western Blot data of Rbx1 expression in HeLa, MCF7, Jurkat and Hep G2 whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn