RBM9 Antibody (F-8): sc-271407

RBM9 Antibody (F-8) is a mouse monoclonal IgG3 antibody that detects RBM9 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RBM9 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RBM9, also known as RNA binding motif protein 9, RTA, fxh, FOX2, Fox-2, HNRBP2, or HRNBP2, is a 390 amino acid protein characterized by the presence of one RNA recognition motif (RRM) domain. RBM9 plays a crucial role in regulating alternative exon splicing, particularly in the nervous system and other cell types, by modulating the splicing activity of the highly conserved RNA 5′-UGCAUGU-3′ element, which serves as an intron splicing enhancer often found adjacent to tissue-specific alternative exons. RBM9 prevents U2AF65 binding, a large subunit of the U2 small nuclear ribonucleoprotein auxiliary factor, to the 3′ splice site of the RNA splicing element, thereby influencing the alternative splicing of tissue-specific exons. Additionally, RBM9 interacts with estrogen receptor alpha (ERα) transcription factor, regulating transcriptional activity, and exists in eight isoforms due to alternative splicing events, highlighting complexity and importance in gene expression regulation.

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RBM9 Antibody (F-8) References:

1. RGS9 proteins facilitate acute tolerance to mu-opioid effects.  |  Garzón, J., et al. 2001. Eur J Neurosci. 13: 801-11. PMID: 11207815
2. Dependence of RGS9-1 membrane attachment on its C-terminal tail.  |  He, W., et al. 2001. J Biol Chem. 276: 48961-6. PMID: 11677233
3. The splicing regulatory element, UGCAUG, is phylogenetically and spatially conserved in introns that flank tissue-specific alternative exons.  |  Minovitsky, S., et al. 2005. Nucleic Acids Res. 33: 714-24. PMID: 15691898
4. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals.  |  Underwood, JG., et al. 2005. Mol Cell Biol. 25: 10005-16. PMID: 16260614
5. Fox-2 splicing factor binds to a conserved intron motif to promote inclusion of protein 4.1R alternative exon 16.  |  Ponthier, JL., et al. 2006. J Biol Chem. 281: 12468-74. PMID: 16537540
6. Role for Fox-1/Fox-2 in mediating the neuronal pathway of calcitonin/calcitonin gene-related peptide alternative RNA processing.  |  Zhou, HL., et al. 2007. Mol Cell Biol. 27: 830-41. PMID: 17101796
7. Regulated Fox-2 isoform expression mediates protein 4.1R splicing during erythroid differentiation.  |  Yang, G., et al. 2008. Blood. 111: 392-401. PMID: 17715393
8. Repression of prespliceosome complex formation at two distinct steps by Fox-1/Fox-2 proteins.  |  Zhou, HL. and Lou, H. 2008. Mol Cell Biol. 28: 5507-16. PMID: 18573872
9. Defining the regulatory network of the tissue-specific splicing factors Fox-1 and Fox-2.  |  Zhang, C., et al. 2008. Genes Dev. 22: 2550-63. PMID: 18794351
10. Functional comparison of RGS9 splice isoforms in a living cell.  |  Martemyanov, KA., et al. 2008. Proc Natl Acad Sci U S A. 105: 20988-93. PMID: 19098104
11. Light-induced translocation of RGS9-1 and Gβ5L in mouse rod photoreceptors.  |  Tian, M., et al. 2013. PLoS One. 8: e58832. PMID: 23555598
12. Nucleus accumbens-specific interventions in RGS9-2 activity modulate responses to morphine.  |  Gaspari, S., et al. 2014. Neuropsychopharmacology. 39: 1968-77. PMID: 24561386

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RBM9 Antibody (F-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RBM9 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RBM9 Antibody (F-8) Specifications

Alternate Product Name: RBM9

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 46-120

Epitope Target: RBM9

Epitope Species: human

Product Reactivity/Detection: RBM9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RBM9 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin