RBM9 Antibody (D-10): sc-365386

RBM9 Antibody (D-10) is a mouse monoclonal IgM antibody that detects RBM9 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RBM9, also known as RNA binding motif protein 9, RTA, fxh, FOX2, Fox-2, HNRBP2, or HRNBP2, is a 390 amino acid protein characterized by the presence of one RNA recognition motif (RRM) domain, which is crucial for regulating alternative exon splicing, particularly in the nervous system and various other cell types. RBM9′s ability to modulate splicing is vital for the generation of diverse protein isoforms, allowing cells to adapt functions in response to different physiological conditions. RBM9 specifically influences the splicing activity of the highly conserved RNA 5′-UGCAUGU-3′ element, an intron splicing enhancer frequently found near tissue-specific alternative exons. By preventing U2AF65 binding, a key component of the spliceosome, to the 3′ splice site of this RNA splicing element, RBM9 plays a significant role in determining the fate of tissue-specific exons. Additionally, RBM9 interacts with estrogen receptor alpha (ERα) transcription factor, thereby regulating transcriptional activity, which is essential for various biological processes, including development and cellular response to hormones. Notably, RBM9 exists in eight isoforms due to alternative splicing events, further underscoring complexity and importance in gene regulation.

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RBM9 Antibody (D-10) References:

1. Cloning and characterization of RGS9-2: a striatal-enriched alternatively spliced product of the RGS9 gene.  |  Rahman, Z., et al. 1999. J Neurosci. 19: 2016-26. PMID: 10066255
2. Specific binding of RGS9-Gbeta 5L to protein anchor in photoreceptor membranes greatly enhances its catalytic activity.  |  Lishko, PV., et al. 2002. J Biol Chem. 277: 24376-81. PMID: 12006596
3. Essential role for RGS9 in opiate action.  |  Zachariou, V., et al. 2003. Proc Natl Acad Sci U S A. 100: 13656-61. PMID: 14595021
4. Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of the R9AP knockout mouse.  |  Keresztes, G., et al. 2004. J Biol Chem. 279: 1581-4. PMID: 14625292
5. Brain-specific RGS9-2 is localized to the nucleus via its unique proline-rich domain.  |  Bouhamdan, M., et al. 2004. Biochim Biophys Acta. 1691: 141-50. PMID: 15110994
6. RGS9-2 modulates D2 dopamine receptor-mediated Ca2+ channel inhibition in rat striatal cholinergic interneurons.  |  Cabrera-Vera, TM., et al. 2004. Proc Natl Acad Sci U S A. 101: 16339-44. PMID: 15534226
7. D2 dopamine receptors colocalize regulator of G-protein signaling 9-2 (RGS9-2) via the RGS9 DEP domain, and RGS9 knock-out mice develop dyskinesias associated with dopamine pathways.  |  Kovoor, A., et al. 2005. J Neurosci. 25: 2157-65. PMID: 15728856
8. Motor coordination deficits in mice lacking RGS9.  |  Blundell, J., et al. 2008. Brain Res. 1190: 78-85. PMID: 18073128
9. RGS9: a regulator of G-protein signalling with specific expression in rat and mouse striatum.  |  Thomas, EA., et al. 1998. J Neurosci Res. 52: 118-24. PMID: 9556034
10. High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods.  |  Cowan, CW., et al. 1998. Proc Natl Acad Sci U S A. 95: 5351-6. PMID: 9560279

The preferred RBM9 antibody is RBM9 Antibody (F-8): sc-271407.