RBM27 Antibody (H-9) recommended for detection of RBM27 of mouse, rat, human origin by WB, IP, IF and ELISA

RBM27 Antibody (H-9): sc-515704

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Datasheets

    • Anti-RBM27 Antibody (H-9) is a mouse monoclonal IgG1 (kappa light chain) RBM27 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 466-489 within an internal region of RBM27 of human origin
    • Anti-RBM27 Antibody (H-9) is recommended for detection of RBM27 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-RBM27 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RBM27 (H-9): sc-515704.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for RBM27 Antibody (H-9) for WB applications. This reagent is now offered in a bundle with RBM27 Antibody (H-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using RBM27 (H-9): sc-515704?

Asked by: Germaine
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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