rBAT Antibody (G-5): sc-518240
- rBAT Antibody (G-5) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 274-295 of rBAT of human origin
- recommended for detection of rBAT of human origin by WB, IP, IF and ELISA
- Anti-rBAT Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for rBAT Antibody (G-5) for WB applications. These reagents are now offered in bundles with rBAT Antibody (G-5) (see ordering information below).
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rBAT Antibody (G-5) is a mouse monoclonal IgG2a kappa light chain antibody specific for an epitope mapping between amino acids 274-295 of rBAT of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). rBAT monoclonal antibody (G-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Neutral and basic amino acid transport protein (rBAT), encoded by the SLC3A1 gene, plays a crucial role in the reabsorption of dibasic amino acids and cystine in renal and intestinal epithelial cells, functioning as a heavy chain subunit of heterodimeric amino acid transporters. rBAT (G-5) antibody is essential for maintaining amino acid homeostasis, and defects in transport system can lead to cystinuria, a condition characterized by the formation of kidney stones due to the accumulation of cystine. rBAT protein dimerizes with one of seven identified light chain subunits, and mutations in either the SLC3A1 gene or the gene encoding the light chain subunit, SLC7A9, can result in cystinuria, classified as "Type I" and "non-type I," respectively. The clinical manifestations of these mutations are indistinguishable, highlighting the importance of the functional complex formed by both subunits for normal amino acid transport. These attributes make anti-rBAT antibody (G-5) an essential tool for investigating amino acid transport mechanisms and the molecular basis of cystinuria.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
rBAT Antibody (G-5) References:
- Non-type I cystinuria caused by mutations in SLC7A9, encoding a subunit (bo,+AT) of rBAT. | Feliubadaló, L., et al. 1999. Nat Genet. 23: 52-7. PMID: 10471498
- Cystine transport activity of heterozygous rBAT mutants expressed in Xenopus oocytes. | Ishihara, M., et al. 2002. Nephron. 91: 276-80. PMID: 12053065
- Influence of rBAT-mediated amino acid transport on cytosolic pH. | Moschèn, I., et al. 2002. Nephron. 91: 631-6. PMID: 12138266
- Cystinuria in children: distribution and frequencies of mutations in the SLC3A1 and SLC7A9 genes. | Botzenhart, E., et al. 2002. Kidney Int. 62: 1136-42. PMID: 12234283
- [Cystinuria update: clinical, biochemical and genetic aspects]. | Orts Costa, JA., et al. 2003. An Med Interna. 20: 317-26. PMID: 12848605
- A mouse model for cystinuria type I. | Peters, T., et al. 2003. Hum Mol Genet. 12: 2109-20. PMID: 12923163
- Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization. | He, D., et al. 2003. J Lipid Res. 44: 2242-9. PMID: 12951368
- The molecular basis of cystinuria: the role of the rBAT gene. | Palacín, M., et al. 1996. Amino Acids. 11: 225-46. PMID: 24178689
- Computational analysis identifies druggable mutations in human rBAT mediated Cystinuria. | Pandey, B., et al. 2021. J Biomol Struct Dyn. 39: 5058-5067. PMID: 32602810
- rBAT, related to L-cysteine transport, is localized to the microvilli of proximal straight tubules, and its expression is regulated in kidney by development. | Furriols, M., et al. 1993. J Biol Chem. 268: 27060-8. PMID: 8262944
- The rBAT gene is responsible for L-cystine uptake via the b0,(+)-like amino acid transport system in a 'renal proximal tubular' cell line (OK cells). | Mora, C., et al. 1996. J Biol Chem. 271: 10569-76. PMID: 8631857
- rBAT is an amino acid exchanger with variable stoichiometry. | Coady, MJ., et al. 1996. J Membr Biol. 149: 1-8. PMID: 8825523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rBAT Antibody (G-5) | sc-518240 | 200 µg/ml | $316.00 | |||
rBAT Antibody (G-5): m-IgG Fc BP-HRP Bundle | sc-551573 | 200 µg Ab; 10 µg BP | $354.00 | |||
rBAT Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-551572 | 200 µg Ab; 40 µg BP | $354.00 | |||
rBAT Antibody (G-5): m-IgG2a BP-HRP Bundle | sc-551574 | 200 µg Ab; 10 µg BP | $354.00 | |||
rBAT Antibody (G-5) AC | sc-518240 AC | 500 µg/ml, 25% agarose | $416.00 | |||
rBAT Antibody (G-5) HRP | sc-518240 HRP | 200 µg/ml | $316.00 | |||
rBAT Antibody (G-5) FITC | sc-518240 FITC | 200 µg/ml | $330.00 | |||
rBAT Antibody (G-5) PE | sc-518240 PE | 200 µg/ml | $343.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 488 | sc-518240 AF488 | 200 µg/ml | $357.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 546 | sc-518240 AF546 | 200 µg/ml | $357.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 594 | sc-518240 AF594 | 200 µg/ml | $357.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 647 | sc-518240 AF647 | 200 µg/ml | $357.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 680 | sc-518240 AF680 | 200 µg/ml | $357.00 | |||
rBAT Antibody (G-5) Alexa Fluor® 790 | sc-518240 AF790 | 200 µg/ml | $357.00 |